Pleas De ad l to o nn oT tr aa nd sj au cs t ti omn sa rgins
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COMMUNICATION
Dalton Transactions
Overall, these findings highlight the influence of the third
coordination sphere on the catalytic performance of the
ferritin-encapsulated ATHases.
Taking advantage of the reversible dissociation and
reassembly of apoferritin, we have compartmentalized an
artificial metalloenzyme within a protein cage. The resulting
ATHases maintained their catalytic activity, displaying up to >
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800 TON for the reduction of cyclic imines. The marked
1
variation in enantioselectivity, observed upon ATHase
encapsulation, highlights the combined effect of both the
second- and third coordination sphere provided by Sav and
ferritin respectively.
These results highlight the possibility to encapsulate an ArM
within ferritin following a reassembly route. The significant
improvement in TON compared to the free ArM suggests that
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metal in a cellular environment and possibly to deliver it in vivo,
without eliciting an immune response.16
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Conflicts of interest
There are no conflicts to declare.
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Acknowledgements
This research was supported by the Swiss Nanoscience Institute
(Project NanoZyme, DPA2238), the ERC (DrEAM) and the NCCR
Molecular Systems Engineering. MRH would like to thank the
Biophysics Facility of the University of Basel for the use of the
DLS instrument.
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