Bio-inspired nitrile hydration by peptidic ligands based on L-cysteine, L-methionine or L-penicillamine and pyridine-2,6-dicarboxylic acid

Article abstract of DOI:10.3390/molecules191220751

Nitrile hydratase (NHase, EC 4.2.1.84) is a metalloenzyme which catalyses the conversion of nitriles to amides. The high efficiency and broad substrate range of NHase have led to the successful application of this enzyme as a biocatalyst in the industrial syntheses of acrylamide and nicotinamide and in the bioremediation of nitrile waste. Crystal structures of both cobalt(III)- and iron(III)-dependent NHases reveal an unusual metal binding motif made up from six sequential amino acids and comprising two amide nitrogens from the peptide backbone and three cysteine-derived sulfur ligands, each at a different oxidation state (thiolate, sulfenate and sulfinate). Based on the active site geometry revealed by these crystal structures, we have designed a series of small-molecule ligands which integrate essential features of the NHase metal binding motif into a readily accessible peptide environment. We report the synthesis of ligands based on a pyridine-2,6-dicarboxylic acid scaffold and L-cysteine, L- S-methylcysteine, L-methionine or L-penicillamine. These ligands have been combined with cobalt(III) and iron(III) and tested as catalysts for biomimetic nitrile hydration. The highest levels of activity are observed with the L-penicillamine ligand which, in combination with cobalt(III), converts acetonitrile to acetamide at 1.25 turnovers and benzonitrile to benzamide at 1.20 turnovers.

Full text of DOI:10.3390/molecules191220751

Molecules 2014, 19, 20751-20767; doi:10.3390/molecules191220751
OPEN ACCESS
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Bio-Inspired Nitrile Hydration by Peptidic Ligands Based on
L-Cysteine, L-Methionine or L-Penicillamine and
Pyridine-2,6-dicarboxylic Acid
Cillian Byrne †, Kate M. Houlihan †, Prarthana Devi, Paul Jensen and Peter J. Rutledge *
School of Chemistry F11, The University of Sydney, Sydney, NSW 2006, Australia;
E-Mails: cilliandbyrne@googlemail.com (C.B.); tynan.kate@gmail.com (K.M.H.);
prarthana.devi@sydney.edu.au (P.D.); paul.jensen.email@gmail.com (P.J.)
†
These authors contributed equally to this work.
* Author to whom correspondence should be addressed; E-Mail: peter.rutledge@sydney.edu.au;
Tel.: +61-293-515-020; Fax: +61-293-513-329.
External Editor: Katrina Jolliffe
Received: 2 September 2014; in revised form: 6 November 2014 / Accepted: 16 November 2014 /
Published: 12 December 2014
Abstract: Nitrile hydratase (NHase, EC 4.2.1.84) is a metalloenzyme which catalyses the
conversion of nitriles to amides. The high efficiency and broad substrate range of NHase
have led to the successful application of this enzyme as a biocatalyst in the industrial
syntheses of acrylamide and nicotinamide and in the bioremediation of nitrile waste. Crystal
structures of both cobalt(III)- and iron(III)-dependent NHases reveal an unusual metal
binding motif made up from six sequential amino acids and comprising two amide nitrogens
from the peptide backbone and three cysteine-derived sulfur ligands, each at a different
oxidation state (thiolate, sulfenate and sulfinate). Based on the active site geometry revealed
by these crystal structures, we have designed a series of small-molecule ligands which
integrate essential features of the NHase metal binding motif into a readily accessible peptide
environment. We report the synthesis of ligands based on a pyridine-2,6-dicarboxylic acid
scaffold and L-cysteine, L-S-methylcysteine, L-methionine or L-penicillamine. These ligands
have been combined with cobalt(III) and iron(III) and tested as catalysts for biomimetic
nitrile hydration. The highest levels of activity are observed with the L-penicillamine ligand
which, in combination with cobalt(III), converts acetonitrile to acetamide at 1.25 turnovers
and benzonitrile to benzamide at 1.20 turnovers.

Molecules 2014, 19
Keywords: nitrile hydratase; peptidomimetics; metalloenzyme; amino acid
20752
1. Introduction
The development of mild, environmentally benign methods to convert organic nitriles to primary
amides (Figure 1a) is of considerable interest given potential applications of these methods in synthesis,
bioremediation and the chemical industry [1–3]. Catalytic hydration of nitriles offers an efficient route
to amides, which have a wide range of potential applications in polymer manufacture and pharmaceutical
synthesis [1,2].
Classical methods for hydrating nitriles require treatment with strong acid or base under forcing
conditions which often result in over-hydrolysis to the carboxylic acid [3]. A number of promising
systems have been developed in recent years using transition metal catalysis to effect nitrile hydration
under increasingly mild and selective conditions [1,2]. Of particular note are recent approaches
employing ruthenium [4–7], rhodium [8,9], palladium [10,11], platinum [12,13], copper [14], silver [15] and
gold catalysts [16,17]. Transition metal-free strategies have also been reported recently using potassium
carbonate [18] or 1,3-dimethylimidazolium hydrogen carbonate as an organocatalyst [19], and
anhydrous conversion of nitriles to amides has been achieved using aldoximes as the water
source [11,20,21].
Figure 1. (a) Nitrile hydration to the primary amide (and onward to carboxylic acid and
ammonia); (b) The metal binding environment at the active site of Co-NHase [22]. The metal
is coordinated by two nitrogen atoms from backbone amides and three sulfur atoms; all the
S-ligands derive from the side-chain thiols of cysteine residues, but each is in a different
oxidation state: thiolate (blue), sulfenate (green) and sulfinate (red). X = solvent.
Biocatalysis offers another efficient route to amides from nitriles [23,24]. The hydro-lyase enzyme
nitrile hydratase (NHase, EC 4.2.1.84) is used in the industrial production of nicotinamide and
acrylamide [24,25], and is increasingly utilised as a biocatalyst for synthesis [26]. NHase has been
well studied, and a variety of mechanistic investigations and high-resolution crystal structures
reported [22,23,27,28]. NHase is a metalloenzyme and incorporates iron(III) or cobalt(III) in an unusual
coordination sphere (Figure 1b). It was long thought that the active site metal ion either activated water
to attack the nitrile or activated the nitrile to attack by water [29,30]. However recent structural studies

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by Holz and co-workers [31] and theoretical calculations by Hopman and Himo [32,33] suggest that the
cysteine-sulfenic acid ligand is the catalytic nucleophile.
Several groups have used NHase as the inspiration for biomimetic nitrile hydration [29,30,34–40]. A
handful of synthetic small-molecule complexes incorporating nitrogen and sulfur ligands have been
shown to bind nitriles [41] and to promote nitrile hydration in solution [4,35,37,39]. Building on these
previous biomimetic approaches to nitrile hydration and our work with related non-heme iron
systems [42–47], we report now the synthesis and evaluation of ligands 1–4 based on the pyridine-2,6-
dicarboxylic acid scaffold 5 (Figure 2) and the amino acids L-cysteine (6), L-S-methylcysteine (7),
L-methionine (8) and L-penicillamine (9). These compounds introduce a peptide character around the
pyridine-2,6-dicarboxamide core to furnish pentadentate N3S2 ligands to mimic the NHase active site.
Figure 2. Ligands 1–4 prepared and studied in the current investigation are based on
pyridine-2,6-dicarboxylic acid 5 and sulfur-containing amino acids.
2. Results and Discussion
2.1. Synthesis of Ligands
Ligands 1–4 were prepared from pyridine-2,6-dicarboxylic acid (5) and L-cysteine (6), L-methionine (8)
and L-penicillamine (9) (Scheme 1). The amino acid constituents were derivatised using established
procedures for the preparation of S-methyl-L-cysteine [48] and S-p-methoxybenzyl-L-cysteine [49] from
L-cysteine (6), protection of L-penicillamine (9) via the S-p-methoxybenzyl (PMB) derivative 10 [49], and
methyl esterification [50,51]. The methyl esters 11–14 were then coupled to the diacid 5 using
N,N,N′,N′-tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU) under standard
conditions [52]. This afforded ligands 2 and 3 directly and the S-protected forms of ligands 1 and 4, the
S-p-methoxybenzyl-L-cysteine derivative 15 and S-p-methoxybenzyl-L-penicillamine analogue 16.
Trifluoroacetic acid-mediated deprotection using triisopropylsilane (TIS) as a cation scavenger was used
to convert 15 to ligand 1 and 16 to ligand 4 [49]. The methionine derivative 3 has previously been reported
by Deardau et al., who investigated the use of this compound and the pyridine N-oxide derivative as a
ligand for enantioselective reduction of ketones [53], while compound 1 has been described briefly
(without full characterisation details) in a report detailing its use in the preparation of hybrid membranes
that incorporate amide receptors [54].

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Scheme 1. Synthesis of ligands 1–4 from pyridine-2,6-dicarboxylic acid (5) and the amino
acids
L-cysteine (6),
L
-S-methylcysteine (7),
L
-methionine (8) and
L
-penicillamine (9).
SPMB
OMe
H2N
i,ii
x,xi
1
O
SH
OH
11
H2N
SMe
O
6
iii,iv
xii
OMe
2
HCl.H2N
O
12
SMe
SMe
v
xiii
3
OH
OMe
H2N
HCl.H2N
O
8
O
13
SH
SPMB
OH
SPMB
viii, ix
vi, vii
xiv,xv
OH
4
OMe
H2N
BocHN
H2N
O
9
O
10
O
14
Reagents and Conditions: i. PMBCl, TFA, DCM, 0 °C 1 h then rt 18 h, 93%; ii. SOCl2, MeOH, reflux,
20 h, 77%; iii. Na, MeI, EtOH, 0 °C, 30 min, 85%; iv. SOCl2, MeOH, reflux 4 h then rt 16 h, 100%; v.
i
SOCl2, MeOH, rt 4 h then reflux 20 h, 89%; vi. PMBCl, AcOH, 80 °C, 72 h; vii. Boc2O, Et2 PrN,
MeOH, reflux 3 h then rt 16 h, 89% over two steps; viii. NaHCO3, MeI, DMF, rt, 16 h, 62%; ix. p-
toluenesulfonic acid, CH2Cl2, reflux, 3 h then NaHCO3, 90%; x. pyridine-2,6-dicarboxylic acid 5,
HBTU, Et3N, CH2Cl2, rt, 20 h, 42%; xi. TFA, TIS, reflux, 2.5 h, 63%; xii. 5, HBTU, Et3N, CH2Cl2,
i
rt, 23 h, 68%; xiii, 5, HBTU, Et3N, CH2Cl2, 24 h, rt, 33%; xiv, 5, HBTU, Et2 PrN, CHCl3, rt, 24 h
58%; xv. TFA, TIS, reflux, 3.5 h 91%.
Crystallisation of compound 2 from methanol/water afforded one colourless plate (0.32 × 0.27 × 0.08 mm)
from which a single crystal X-ray structure was determined. This structure reveals trans geometry about
both amide bonds, and the S-methyl groups oriented away from the pyridine nitrogen (Figure 3 and
Supporting Information).
Figure 3. X-Ray crystal structure of pyridine-2,6-dicarboxylic acid bis(S-methyl-L-cysteine
methyl ester)carboxamide (2). Carbon atoms are shown in grey, oxygen in red, nitrogen in
lilac, sulfur in yellow and hydrogen in white. CCDC-1017988 contains the full supplementary
crystallographic data for this paper. These data can be obtained free of charge from The
Cambridge Crystallographic Data Centre via http://www.ccdc.cam.ac.uk/data_request/cif.

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2.2. Nitrile Hydration Experiments
The ability of ligands 1–4 to promote nitrile hydration was assessed under a range of conditions
adapted from those reported previously by Chottard and Mascharak to achieve biomimetic nitrile
hydration with small-molecule systems [4,35,37,39]. Thus different permutations of ligand 1–4, metal
ion (iron(II), iron(III), cobalt(II) and cobalt(III) salts), base (to effect ligand deprotonation and moderate
pH), oxidant (to bring about S-oxidation) and nitrile substrate were combined in situ (Table 1). Acetonitrile
17 was used throughout screening experiments, present in excess as both substrate and co-solvent.
Turnover reaction products were analysed by gas chromatography (GC) in comparison to authentic
samples of the starting material 17 and expected product acetamide 18; turnover was quantified using
the single point internal standard method [55,56]. See Supporting Information for details of GC
conditions and representative chromatograms (Supplementary Figures S1–S4).
Table 1. Summary of variables screened in nitrile turnover experiments.
Ligand
Metal Salt
Fe(OAc)2
Temperature (°C)
pH
Oxidant
1
2
3
4
Fe(NO3)3
0
2
5
9
+H2O2
–H2O2
Na3[Co(NO2)6]
CoCl2.6H2O
[Co(NH3)5Cl]Cl2
20
50
Turnover experiments were typically conducted by dissolving the ligand in acetonitrile, treating with
aqueous sodium hydroxide (four equivalents for thiolate ligands 1 and 4 (2 × SH, 2 × NH, anticipating
that the acidity of the amide protons would be increased by N-coordination to the metal); two equivalents
for thioethers 2 and 3) then adding the metal salt to effect complexation. The resulting solution was
adjusted to the required pH using hydrochloric acid or aqueous sodium hydroxide solution, and hydrogen
peroxide (3 equivalents) was added to one series of experiments with a view to achieving S-oxidation in
situ [34,57]. The mixture was then adjusted to the required temperature and stirred for the allotted time
(12–72 h). All turnover reactions were carried out in triplicate in order to obtain consistent results, thus
turnover data discussed below are the average of at least three discrete experiments.
A comprehensive range of control experiments was carried out: (i) no metal salt; (ii) no ligand;
(iii) no base; and (iv) no oxidant, to test for direct nitrile hydration by the free ligand (thiols are known
to increase the rate of nitrile hydrolysis under certain conditions [58]) the metal (since cobalt(III) can
directly hydrolyse nitriles in solution [59]), the added base (to control for direct hydrolysis by hydroxide,
and by thiolate or metal-bound hydroxide species) and the oxidant (to account for the possibility of direct
reaction between nitrile and peroxide). Figure S2 (Supporting Information) shows the chromatogram
arising from a typical control experiment. Turnover was only observed when both metal and ligand were
present (Table 2).
Ligands 1 and 2 returned no nitrile hydration under most of the conditions screened, and only very
low levels of reaction at their best: 0.20 and 0.40 turnovers respectively (i.e., sub-stoichiometric amide
formation) achieved using Na3[Co(NO2)6]/pH 9/no H2O2/50 °C/72 h with 1 and Fe(NO3)3/pH 9/H2O2
(3 eq.)/20 °C/72 h with 2. The control experiments outlined above confirmed that the specific

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combinations of ligand, metal, base and oxidant (in the case of 2) were required to mediate turnover.
The methionine ligand 3 did not turn over acetonitrile under any of the conditions tested.
Table 2. Summary of optimal conditions for acetonitrile turnover with ligands 1–4.
Ligand
Optimal Turnover Conditions
Turnovers
0.20
1
2
3
4
Na3[Co(NO2)]6
Fe(NO3)3
–
50 °C
20 °C
–
pH 9
pH 9
–
72 h
72 h
–
−H2O2
+H2O2
–
0.40
–
Na3[Co(NO2)]6
25 °C
pH 9
12 h
−H2O2
1.25
Ligand 4 returned the best turnover results found with these systems, when combined with
Na3[Co(NO2)6] and stirred for 12 h at 25 °C and pH 9 (Scheme 2 shows the reaction outcome,
Figure S3 (Supporting Information) shows a representative chromatogram for this level of turnover).
The geminal dimethyl unit adjacent to the thiolate enhances the sub-stoichiometric turnover seen with 1
to a gently catalytic level of 1.25 turnovers. The addition of peroxide diminishes catalytic activity with
this ligand (down to 0.7 turnovers), while increasing reaction temperature to 50 °C also lowers activity
to 0.7 turnovers.
Scheme 2. Optimised conditions for nitrile hydration using ligand 4.
To investigate the scope of this ligand, a further set of experiments was carried out using a second
substrate, the aromatic nitrile benzonitrile 19. Under these conditions the combination of ligand 4 and
Na3[Co(NO2)6 also turned over benzonitrile 19 as a substrate, with a similar level of conversion to
benzamide 20 (1.2 turnovers). (To aid the miscibility of the benzonitrile in water, a small amount of
DMF was added to these reactions: 200 µL in a total reaction volume of 7.2 mL).
3. Experimental Section
3.1. General Information
Chemicals were supplied by Sigma-Aldrich (Castle Hill, NSW, Australia), Merck Millipore
(Bayswater, Vic, Australia) or Bio-Scientific (Kirrawee, NSW, Australia). All reactions were carried out
under a dry nitrogen or argon atmosphere unless otherwise indicated. Analytical thin-layer chromatography
(TLC) was performed on Merck Kieselgel 0.25 mm silica gel 60 F254 plates (Merck Millpore).
TLC plates were visualised using UV fluorescence at 254 nm, or by staining with Goofy’s reagent
(6:6:194:1 phosphomolybdic acid–conc. sulphuric acid–water–cerium sulfate). Flash chromatography
on silica gel was performed using a force flow of the indicated solvent system on Ajax Finechem silica
gel (230–400 mesh).

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Solvents used for extraction and chromatography were distilled prior to use. Where necessary,
solvents and reagents were dried over 4 Å molecular sieves prior to use. Tetrahydrofuran was distilled
anaerobically from sodium wire. Dichloromethane, methanol, acetonitrile, diisopropylethylamine and
triethylamine were distilled from calcium hydride. Dimethylformamide was distilled and stored over
4 Å molecular sieves. Methyl iodide was purified by passage through neutral alumina. Anisole and
benzonitrile and were purified by distillation under reduced pressure. Benzamide was recrystallised from
water. All other commercial reagents were used as received without further purification.
Melting points were recorded on a Gallenkamp melting point apparatus (Sanyo Gallenkamp,
1
13
Loughborough, UK). H- and C-NMR spectra were recorded on Bruker Avance DPX200, DPX300
and DPX400 spectrometers (Bruker, Billerica, MA, USA). Chemical shifts (δ) are reported in ppm from
tetramethylsilane (TMS), using TMS or the solvent resonance as the internal reference. Low resolution
mass spectra were obtained on a Finnigan LCQ MS Detector (ESI, APCI) (Thermo Fisher Scientific,
Waltham, MA, USA). High resolution mass spectra were obtained on a Fisons VG Tofspec (MALDI-
TOF) (Thermo Fisher Scientific). Optical rotations were measured on a Bio-Rad FTS-40 polarimeter
(Bio-Rad Laboratories Inc., Hercules, CA, USA) with sodium lamp operating at 589 nm using a 1 dm
cell and concentrations (c) measured in 100 mg/mL. IR spectra were measured at room temperature on
a Bio-Rad Shimadzu 8400S (Bio-Rad Laboratories Inc.) using a sodium chloride cell, NaCl plates or
KBr disc. Wavenumbers are given in reciprocal cm and bands are expressed as strong (s), medium (m)
and weak (w). Gas chromatography was carried out on a Hewlett Packard 5890A gas chromatograph
(Hewlett Packard, Palo Alto, CA, USA). The column used was a SGE 25QC2/BPX with an internal
diameter of 0.25 nm.
3.2. Preparation of Protected Amino Acids
General Methyl Esterification Procedure [50]
Amino acids/protected amino acids were treated with thionyl chloride (2 equivalents) in methanol,
heated at reflux for 4 h and then stirred for 16 h at room temperature. The solvent was removed
in vacuo and the crude product recrystallized from diethyl ether to yield the methyl ester as its
hydrochloride salt in high yield and high purity.
S-p-Methoxybenzyl-L-cysteine methyl ester (11) [49,50]. L-Cysteine (6, 5.61 g, 46.3 mmol) was dissolved
in TFA (15 mL) and DCM (50 mL), cooled to 0 °C and stirred while a solution of
p-methoxybenzyl chloride (7.22 g, 46.1 mmol) in DCM (50 mL) was added dropwise. The reaction
mixture was stirred at 0 °C for 1 h then allowed to warm to room temperature and stirred for 18 h.
Methanol (20 mL) was added and the product was extracted into water (2 × 70 mL). The aqueous phase
was washed with DCM (70 mL) and solid impurities removed by filtration. The filtrate was concentrated
in vacuo to afford S-p-methoxybenzyl-L-cysteine trifluoroacetate salt as a white solid (15.24 g, 93%);
this intermediate (15.24 g, 42.9 mmol) was dissolved in methanol (100 mL) and thionyl chloride (5.0 mL,
68.5 mmol) added drop-wise. The reaction mixture was heated at reflux for 18 h before the solvent was
removed in vacuo to yield an off-white solid. This crude product was partitioned between ethyl acetate
(40 mL) and saturated aqueous NaHCO3 (20 mL). The organic phase was washed with a further aliquot
of saturated aqueous NaHCO3 (20 mL), water (40 mL) and brine (40 mL) then dried (MgSO4). The

Molecules 2014, 19
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solvent was removed in vacuo to yield 11 as viscous brown oil (8.42 g, 77%); νmax (thin film): 3364,
2950, 2837, 2363, 1734, 1643, 1551, 1450, 1300; δH (CDCl3, 300 MHz) 2.70 (1H, dd, J = 13.5, 7.5),
2.84 (1H, dd, J = 13.5, 4.5), 3.70 (2H, s), 3.72 (3H, s), 3.79 (3H, s), 4.62–4.68 (1H, m), 5.31 (1H, br s),
6.84 (2H, d, J = 8.5), 7.22 (2H d, J = 8.5); δC (CDCl3, 75 MHz) 36.0, 36.3, 52.7, 54.0, 55.3, 114.0, 130.0,
150.2, 158.7, 174.4; m/z (ESI+) 256 (100%, [M+H]+). Data in agreement with literature [60].
S-Methyl-L-cysteine methyl ester hydrochloride salt (12) [48]. L-Cysteine (6, 4.20 g, 35.0 mmol) was
dissolved in absolute ethanol (90 mL). Sodium metal (2.75 g, 120.0 mmol) was added slowly to the
solution at 0 °C. The reaction mixture was allowed to stir for a further 30 min. Methyl iodide
(2.10 mL, 26.0 mmol, purified by passing down a short column of alumina prior to use) was added drop-
wise to the reaction at 0 °C. The mixture was allowed to warm to room temperature over a period of 30
min. The solution was adjusted to pH 5 using HCl (10 M) before diethyl ether (80 mL) was added,
affording a cloudy white suspension. The mixture was filtered under vacuum and the isolated solid was
washed with ether (100 mL) and dried under vacuum to give S-methyl-L-cysteine (4.00 g, 85%). A
portion of this solid (0.56 g, 4.40 mmol) was subjected to the general methyl esterification conditions
using thionyl chloride (0.65 mL, 8.80 mmol) in methanol (10 mL) to give the title compound 12 as a
white solid (0.82 g, 85%); νmax (KBr pellet): 3362 (s), 1745 (s), 1606 (s); δH (300 MHz, D2O): 2.10 (3H,
s), 3.01 (1H, dd, J = 15.0, 7.5 Hz), 3.13 (1H, dd, J = 15.0, 4.5 Hz), 3.81 (3H, s), 4.30 (1H, dd, J = 7.5,
4.5 Hz); δC (75.4 MHz, CDCl3): 16.2, 34.1, 52.7, 53.6, 168.8; m/z (ES+): 150 (15%, [M+H]+). Data in
agreement with literature [61].
L-Methionine methyl ester hydrochloride salt (13) [50]. Using the general methyl esterification conditions,
L-methionine (8, 2.00 g, 13.4 mmol) was treated with thionyl chloride (2.00 mL, 26.8 mmol) in methanol
(20 mL) to afford the title compound as a white powder (1.95 g, 89%); νmax (KBr pellet): 3080 (s), 1747
(s), 2840 (w), 1236 (s); δH (300 MHz, CDCl3): 2.28 (3H, s), 2.28–2.33 (2H, m), 2.67–2.77 (2H, m), 3.87
(3H, s), 4.16–4.30 (1H, m); δC (75.4 MHz, CD3OD): 15.4, 30.5, 31.1, 53.2, 54.2, 171.2; m/z (ES+): 164
(100%, [M+H]+). Data in agreement with literature [62].
S-p-Methoxybenzyl-L-penicillamine methyl ester (14) [51]. To a solution of L-penicillamine (9, 0.25 g,
1.60 mmol) in glacial acetic acid (5.0 mL) was added p-methoxybenzyl chloride (0.24 mg, 227 μL, 1.55
mmol). The resulting mixture was heated at 80 °C for 72 h then the solvent was removed in vacuo. The
oily residue was dissolved in acetonitrile (5.0 mL) and the solvent was removed in vacuo. This procedure
was repeated twice more to reveal a white solid. This solid was dissolved in a solution of
diisopropylethylamine in methanol (10% v/v, 12.0 mL) and stirred. Di-tert-butyl-dicarbonate (0.71 g,
3.25 mmol) was added in a single portion. The reaction mixture was heated at reflux for 3 h and then
left to stir at room temperature overnight. The solvent was removed in vacuo and the oily residue was
dissolved in ethyl acetate (50 mL). This solution was washed with aqueous HCl (1 M, 40 mL), water (40
mL) and brine (40 mL), then dried over MgSO4, and the solvent was removed in vacuo to reveal N-tert-
butyloxycarbonyl-S-p-methoxybenzyl-L-penicillamine (10) as a white solid (0.53 g, 89%); δH (300 MHz,
CD3CN): 1.34 (3H, s), 1.41 (3H, s), 1.42 (9H, s), 3.77 (3H, s), 3.73–3.75 (2H, m), 4.20 (1H, br d, J =
8.5 Hz), 5.62 (1H, br d , J = 8.5 Hz), 6.85 (2H, d, J = 8.5 Hz), 7.23 (2H, d, J = 8.5 Hz); δC (75.4 MHz,
CD3CN): 25.6, 27.1, 28.6, 33.1, 48.1, 56.2, 61.2, 80.2, 114.7, 130.6, 131.2, 159.8, 168.2, 172.2; m/z (ES−):

Molecules 2014, 19
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368 ([M–H]−, 90%), 737 ([2M–H]−, 100%). Compound 10 (1.55 g, 4.21 mmol) and NaHCO3 (0.71 g, 8.40
mmol) were dissolved in DMF (20 mL) and stirred while methyl iodide (1.20 g, 524 μL, 8.40 mmol)
was added. The mixture was stirred overnight. The solvent was removed in vacuo and the crude residue
redissolved in ethyl acetate (20 mL) and washed with saturated aqueous NaHCO3 (10 mL), water (10 mL)
and brine (10 mL). The organic phase was dried over MgSO4 and the solvent removed in vacuo. The
residue was combined with p-toluenesulfonic acid (0.54 g, 4.20 mmol) in dichloromethane (25 mL) and
heated at reflux for 4 h. The resulting solution was cooled to room temperature then washed with
saturated aqueous NaHCO3 (10 mL), water (10 mL) and brine (10 mL). The organic phase was dried
over MgSO4 and evaporated in vacuo to yield the title compound as a clear oil (0.70 g, 59%); [α]2D0 =
+90.4 (c = 0.66 in EtOAc); νmax (thin film) 3378 (s), 1740 (s), 834 (s); δH (300 MHz, CDCl3): 1.29 (3H,
s), 1.42 (3H, s), 1.77 (2H, s), 3.49 (1H, s), 3.71 (1H, s), 3.73 (3H, s), 3.76 (1H, s), 3.78 (3H, s), 6.83 (2H,
d, J = 5.5 Hz), 7.23 (2H, d, J = 5.5 Hz); δC (75.4 MHz, CDCl3): 24.0 26.6, 32.8, 50.2, 51.9, 55.4, 61.8,
110.8, 114.2, 130.2, 159.0, 173.9; m/z (ES+): 284 ([M+H]+, 100%); HRMS (ES+): [M+H]+ C14H22NO3S
requires 284.1315, found 284.1325.
3.3. Peptide Coupling Method
Amino acid methyl ester (as free amine or HCl salt, 0.2–1.8 g, 2 eq.) pyridine-2,6-dicarboxylic acid
(5, 1 eq.) and N,N,N′,N′-tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU,
2 eq.) were dissolved in DCM or chloroform (20–40 mL) and triethylamine or DIPEA (2 eq. for free
amine, 4 eq. for HCl salt) was added. The reaction mixture was stirred at room temperature for 22–48 h
while monitored by TLC. Additional DCM or chloroform (10–20 mL) was added and the solution
washed with equivalent volumes of water, 1 M hydrochloric acid, saturated aqueous sodium hydrogen
carbonate, and brine. The organic phase was dried over MgSO4 then concentrated in vacuo to give the
crude product (generally a yellow oil) which was purified by column chromatography.
3.4. Ligand Synthesis
Pyridine-2,6-dicarboxylic acid bis(S-p-methoxybenzyl-L-cysteine methyl ester) carboxamide (15).
Pyridine-2,6-dicarboxylic acid (5, 0.06 g, 0.39 mmol) and S-p-methoxybenzyl-L-cysteine methyl ester
(11, 0.20 g, 0.78 mmol) were coupled to give 15 as a thick, colourless oil (0.21 g, 42%) after purification
by column chromatography (EtOAc‒petroleum ether 2:1); Rf 0.30 (EtOAc‒petroleum ether 2:1); [α]2D0 =
−44.8 (c = 0.5, CHCl3); νmax (CHCl3): 3501 (w), 1742 (s), 1736 (s); δH (300 MHz, CDCl3): 3.01 (4H, d,
J = 5.5 Hz), 3.62–3.84 (16H, overlapping m and 2 × s), 4.96 (2H, dd, J = 18.5, 7.5 Hz), 6.77 (4H, d, J =
8.5 Hz), 7.20 (4H, d, J = 8.5 Hz), 8.05 (1H, t, J = 7.5 Hz), 8.35 (2H, d, J = 7.5 Hz), 8.57 (2H, d, J = 7.5
Hz); δC (75.4 MHz, CDCl3): 33.2, 36.0, 51.7, 52.7, 55.2, 113.9, 125.3, 129.4, 130.0, 139.1, 148.2, 158.7,
163.1, 171.0; m/z HRMS (ES+): C31H35N3O8S2Na+ ([M+Na]+) requires 664.1763, found 664.1761.
Pyridine-2,6-dicarboxylic acid bis(S-methyl-L-cysteine methyl ester)carboxamide (2). Pyridine-2,6-
dicarboxylic acid (5, 0.23 g, 1.4 mmol) and S-methyl-L-cysteine methyl ester hydrochloride salt (12,
0.50 g, 2.7 mmol) were combined to give 2 as a pale yellow oil (0.39 g, 68%) after purification by
column chromatography (EtOAc‒cyclohexane 2:1); Rf 0.40 (EtOAc‒cyclohexane 2:1); [α]2D0 = −8.0
(c = 1.0, CHCl3); νmax (thin film) 3354 (s), 3043 (w), 1743 (s), 1678 (s); δH (300 MHz, CDCl3): 2.19

Molecules 2014, 19
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(6H, s), 2.84 (4H, d, J = 3.5 Hz), 3.64 (6H, s), 5.02 (2H, dt, J = 8.0, 5.5 Hz), 8.07 (1H, t, J = 8.0 Hz),
8.37 (2H, d, J = 8.0 Hz), 8.64 (2H, d J = 8.0 Hz); δC (75.4 MHz, CDCl3): 16.1, 36.4, 51.6, 52.8, 125.8,
139.5, 148.7, 163.6, 171.4; m/z (ES+): 452 (30%, [M+Na]+), 430 (100%, [M+H]+); HRMS (ES+):
C17H24N3O6S2 ([M+H]+) requires 430.1107, found 430.1118.
Pyridine-2,6-dicarboxylic acid bis(L-methionine methyl ester)carboxamide (3). Pyridine-2,6-dicarboxylic
acid (5, 0.92 g, 5.5 mmol) and L-methionine methyl ester hydrochloride salt (13, 1.80 g, 11.0 mmol)
were united to give 3 as a yellow oil (0.81 g, 33%) after purification by column chromatography (DCM:
EtOAc 4:1); Rf 0.30 (DCM: EtOAc 4:1); [α]2D0 = −10.3 (c = 1.0, CHCl3); νmax (thin film): 3392 (s), 1739
(s), 1677 (s); δH (300 MHz, CDCl3): 2.08 (6H, s), 2.12–2.24 (4H, m), 3.05–3.16 (4H, m), 3.74 (6H, s),
4.87–4.89 (2H, m), 7.92–8.02 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 7.5 Hz), 8.57 (2H, d,
J = 8.0 Hz); δC (75.4 MHz, CDCl3): 15.7, 30.6, 31.5, 52.3, 52.9, 125.9, 139.1, 147.0, 163.7, 172.9;
m/z (ES+): 458 (100%, [M+H]+); HRMS (ES+): C19H28N3O6S2 ([M+H]+) requires 458.1417, found
458.1409. Data in agreement with literature [54].
Pyridine-2,6-dicarboxylic acid bis(S-p-methoxybenzyl-L-penicillamine methyl ester)
carboxamide (16).
Pyridine-2,6-dicarboxylic acid (5, 0.16 g, 0.97 mmol) and S-p-methoxybenzyl-L-penicillamine methyl
ester (14, 0.55 g, 1.94 mmol) were joined to give 16 as a clear yellow oil (0.39 g, 58%) after purification
by column chromatography (hexane‒EtOAc 6:4); Rf 0.25 (hexane‒EtOAc 6:4); [α]2D0 = +2.1 (c = 1.12,
(KBr): 3401 (m), 1742 (m), 1681 (m
EtOAc); νmax
); δH (300 MHz, CDCl3): 1.26 (6H, s), 1.48 (6H, s),
3.62 (6H, s), 3.73 (2H, s), 3.74 (1H, s), 3.76 (6H, s), 4.75 (2H, d, J = 7.5 Hz), 6.71 (4H, d, J = 8.5 Hz),
7.18 (4H, d, J = 8.5 Hz), 8.05 (1H, t, J = 7.5 Hz), 8.35 (2H, d, J = 8.0 Hz), 8.62 (2H, d, J = 7.5 Hz);
δC (75.4 MHz, CDCl3): 25.0, 27.7, 33.4, 48.8, 52.8, 55.6, 60.5, 114.6, 126.3, 129.3, 131.1, 139.2, 148.3,
+
160.0, 164.8, 171.5;
HRMS (ES+): C35H44N3O8S2 ([M+H]+) requires
m/z (ES+): 720 ([M+Na] , 100%);
698.2587, found 698.2595.
Pyridine-2,6-dicarboxylic acid bis(L-cysteine methyl ester)carboxamide (1). Pyridine-2,6-dicarboxylic
acid bis(S-p-methoxybenzyl-L-cysteine methyl ester)carboxamide (15, 100 mg, 0.15 mmol) and
triisopropylsilane (TIS, 195 μL, 0.95 mmol) were dissolved in TFA (1.25 mL). The reaction mixture
was heated at reflux for 2.5 h after which time the solvent was removed in vacuo. The residue was
washed with ether (3 × 2 mL). The crude product was purified by flash column chromatography
(EtOAc‒petroleum ether 2:1) to afford the pure product 1 as white thick oil (41 mg, 63%) which was
stored and handled under nitrogen to minimize disulfide formation; Rf 0.20 (ether: hexane 1:1);
[α]2D0 = +8.0 (c = 0.1, CHCl3); νmax (thin film): 3394 (s), 2570 (w), 1739 (s), 1670 (s); δH (300 MHz,
CDCl3): 1.55 (2H, t, J = 9.0 Hz), 3.14–3.20 (4H, m), 3.84 (6H, s), 5.07–5.09 (2H, m), 8.08 (1H, t,
J = 7.5 Hz), 8.37 (2H, d, J = 7.5 Hz), 8.66 (2H, d, J = 7.0 Hz); δC (75.4 MHz, CDCl3): 27.0, 52.9, 53.6,
125.5, 139.3, 148.2, 163.1, 170.1; m/z (ES+): 402 (100%, [M+H]+), 424 (25%, [M+Na]+); HRMS (ES+):
C15H20N3O6S2 ([M+H]+) requires 402.0791, found 402.0795.
Pyridine-2,6-
Pyridine-2,6-dicarboxylic acid bis-(L-penicillamine methyl ester) carboxamide (4).
dicarboxylic acid bis-S-p-methoxybenzyl-L-penicillamine methyl ester (16, 0.40 g, 0.57 mmol) and
TIS (720 µL, 3.5 mmol) were dissolved in TFA (5.0 mL). The reaction mixture was heated at reflux for
2.5 h. The solvent was removed in vacuo and the residue triturated with ether and hexane. Flash column

Molecules 2014, 19
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chromatography of the crude material (hexane‒EtOAc 3:2) furnished 4 as a clear yellow oil (0.24 g,
91%); Rf 0.30 (hexane‒EtOAc 3:2); [α]2D0 = +2.1 (c = 0.04, EtOAc); νmax
δH
(CHCl3): 1743 (s), 1667 (s);
(300 MHz, CDCl3): 1.52 (6H, s), 1.62 (6H, s), 2.22 (2H, br s), 3.82 (6H, s), 4.83 (2H, d, J = 9.5 Hz),
8.06 (1H, t, J = 8.0 Hz), 8.34 (2H, d, J = 8.0 Hz), 8.75 (2H, d, J = 9.5 Hz); δC (75 MHz, CDCl3): 29.8,
30.8, 46.7, 52.3, 61.0, 125.6, 139.31, 148.2, 163.2, 170.4; m/z (ES+): 480 (10%, [M+Na]+); HRMS
(ES+): C19H27N3NaO6S2 ([M+Na]+) requires 480.1237, found 480.1238.
3.5. Crystallography
A colourless plate-like crystal of 2 was attached with Exxon Paratone N, to a short length of fibre
supported on a thin piece of copper wire inserted in a copper mounting pin. The crystal was quenched
in a cold nitrogen gas stream from an Oxford Cryosystems Cryostream (Oxford Cryosystems Ltd, Long
Hanborough, UK). A Bruker-Nonius FR591 Kappa APEX II diffractometer (Bruker Analytical X-ray
Instruments Inc., Madison, WI, USA) employing graphite monochromated MoKα radiation generated
from a fine-focus rotating anode was used for the data collection. Cell constants were obtained from a
least squares refinement against 7789 reflections located between 5.68 and 65.16° 2θ. Data were
collected at 150(2) Kelvin with ϕ and ω scans to 65.18° 2θ. The data integration and reduction were
undertaken with SAINT (Bruker Analytical X-ray Instruments Inc.) and XPREP (Bruker Analytical X-
ray Instruments Inc.) [63], and subsequent computations were carried out with the X-Seed graphical user
interface (University of Missouri, Columbia, MO, USA) [64]. An empirical absorption correction
determined with SADABS (University of Göttingen, Göttingen, Germany) was applied to the data [65,66].
The structure was solved in the space group P1(#1) by direct methods with SHELXS-97 (University
of Göttingen), and extended and refined with SHELXL-97 [67]. The non-hydrogen atoms in the
asymmetric unit were modelled with anisotropic displacement parameters. A riding atom model with
group displacement parameters was used for the hydrogen atoms. The absolute structure was established
with the Flack parameter [68–71] refining to −0.01(3).
3.6. Turnover Experiments
3.6.1. General Procedure
The ligand (0.10 mmol) was dissolved in the nitrile (5.0 mL) and sodium hydroxide (2–4 eq.,
0.20–0.40 mmol) was added. The metal salt (0.10 mmol) was added and the mixture stirred at ambient
temperature for 15 min. If oxidant was to be included, the solution was cooled to 0 °C while H2O2 (30%,
0.30 mmol) was added dropwise with stirring, and the mixture was stirred on ice for 30 min. Water (2.0 mL)
was added and the mixture adjusted to the required pH (2, 5 or 9, using aqueous HCl or NaOH) and
temperature (0, 20 or 50 °C) then stirred for 12–72 h. The solvent was removed in vacuo, the residue re-
dissolved in acetonitrile (1 mL) and the solution filtered through a plug of silica to remove particulates.
The sample was analysed by gas chromatography against an internal standard, and product(s) were
identified by comparison to authentic samples.

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3.6.2. GC Analysis
Calibration of the internal standard/ relative response factor was carried out using the single point
internal standard method [55,56]. Thus equimolar quantities of anisole and acetamide in ethyl acetate (1 μL)
were injected into the GC and the peak area for each was recorded. Three injections were made and an
average of the three measurements taken. Under the conditions used (Table S1, Supporting Information)
residual acetonitrile 17 eluted with the solvent front. Anisole, the internal standard, eluted with a retention
time (Rt) of 6.88 ± 0.03 min, while the product acetamide 18 eluted at Rt 12.64 ± 0.06 min (Figure S1). The
relative response factor (the relative peak area of the two components) was 3.35 (i.e., the area of the
internal standard was 3.35 times greater than the area of the acetamide product when present in equal
molar quantities). By adding a defined quantity of anisole to turnover reaction mixtures immediately
prior to analysis by GC and using the relative response factor (3.35), the amount of acetamide formed
could be accurately quantified, even at <0.1 turnovers. Further details of GC run times and temperatures,
calculation of internal response factors and example chromatograms are provided in the Supporting
Information (Tables S1 and S2, Figures S1–S4).
4. Conclusions
We have prepared a series of pentadentate ligands that combine sulfur-containing amino acids on a
pyridine-2,6-dicarboxylic acid scaffold to render an N3S2 donor set incorporating key elements of the
NHase active site. Evaluating the iron(III)- and cobalt(III)-complexes of these ligands as catalysts for
nitrile hydration demonstrates low levels of turnover (1.20–1.25 turnovers) for the penicillamine
ligand 4 in combination with cobalt(III), with both acetonitrile and benzonitrile substrates. Placed in
context, the most active small-molecule mimics of NHase catalysis to date employ preformed cobalt
complexes under optimised conditions to achieve 15–18 turnovers for a non-oxidised pyridine dithiolate
ligand (at 50 °C and pH 9.5 in Tris buffer) [35], and 50 turnovers with a bis-sulfenate ligand (at 4 °C
and pH 4.8 in acetate buffer) [37]. Future work will investigate the importance of the pyridine nitrogen
to the catalytic activity of ligand 4.
Supplementary Materials
Supplementary materials can be accessed at: http://www.mdpi.com/1420-3049/19/12/20751/s1.
Acknowledgments
We thank Kelvin Picker for assistance with gas chromatography and Keith Fisher for help with mass
spectroscopy. This work was supported by the University of Sydney and the Centre for Synthesis &
Chemical Biology at University College Dublin under the Programme for Research in Third Level
Institutions (PRTLI) administered by the HEA. Prarthana Devi was supported by a Henry Bertie and
Florence Mabel Gritton Postgraduate Research Scholarship and a University of Sydney World
Scholars Award.

Molecules 2014, 19
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Author Contributions
C.B., K.M.H. and P.J.R. conceived and designed the experiments; C.B., K.M.H., P.D. and P.J.
(crystallography) performed the experiments; P.J. (crystallography), C.B., K.M.H., P.D. and P.J.R.
analyzed the data; C.B., K.M.H. and P.J.R. wrote the paper.
Conflicts of Interest
The authors declare no conflict of interest.
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