ChemSusChem p. 3192 - 3203 (2019)
Update date:2022-08-10
Topics:
Tonin, Fabio
Otten, Linda G.
Arends, Isabel W. C. E.
Epimerization of cholic and chenodeoxycholic acid (CA and CDCA, respectively) is a notable conversion for the production of ursodeoxycholic acid (UDCA). Two enantiocomplementary hydroxysteroid dehydrogenases (7α- and 7β-HSDHs) can carry out this transformation fully selectively by specific oxidation of the 7α-OH group of the substrate and subsequent reduction of the keto intermediate to the final product (7β-OH). With a view to developing robust and active biocatalysts, novel NADH-active 7β-HSDH species are necessary to enable a solely NAD+-dependent redox-neutral cascade for UDCA production. A wild-type NADH-dependent 7β-HSDH from Lactobacillus spicheri (Ls7β-HSDH) was identified, recombinantly expressed, purified, and biochemically characterized. Using this novel NAD+-dependent 7β-HSDH enzyme in combination with 7α-HSDH from Stenotrophomonas maltophilia permitted the biotransformations of CA and CDCA in the presence of catalytic amounts of NAD+, resulting in high yields (>90 %) of UCA and UDCA.
View MoreContact:+86-532-80762375
Address:No. 6, Hongkong Middle Road, Qingdao, China
Contact:021-61840961 021-61840962
Address:No.157,Zhuguang Rd, Qingpu, Shanghai, China
Laizhou City Laiyu Chemical CO.,Ltd
Contact:+86-535-2719337/2719339
Address:Chenggang road zhuyou laizhou City Shangdong China
Beijing ZhongDaXinHe Chemical Product Co.,Ltd
Contact:010-52876516
Address:tongzhoubeiyuan
Penglai Qianwei Chemical Co., Ltd.
Contact:86-535-3357802
Address:Shahelu (north), Penglai, Shandong, China
Doi:10.1002/psc.1201
(2010)Doi:10.1246/cl.1992.891
(1992)Doi:10.1016/j.ijpharm.2020.119031
(2020)Doi:10.1134/S1070428015010224
()Doi:10.1021/ja01020a020
(1968)Doi:10.1021/ja01571a070
(1957)