An alternative and convenient strategy for generation of substantial quantities of singly 5′-32p-end-labeled double-stranded DNA for binding studies: Development of a protocol for examination of functional features of (+)-CC-1065 and the duocarmycins that contribute to their sequence-selective DNA alkylation properties

Article abstract of DOI:10.1016/S0040-4020(01)81798-1

Development of an alternative strategy for securing substantial quantities of singly 5′-³²P-end-labeled double-stranded DNA suitable for binding studies is described based on M133 cloning techniques and offers advantages of production of replenishable quantities of singly 5′-³²P-end-labeled double-stranded.