Hepatoprotective aliphatic glycosides from three Goodyera species

Article abstract of DOI:10.1248/bpb.23.731

Hepatoprotective aliphatic glycosides 3-(S)-3-β-D- glucopyranosyloxybutanolide (1) and its congener, 3-(S)-3-β- glucopyranosyloxy-4-hydroxybutanoic acid (2) were isolated as major constituents from the whole plants of three Goodyera species, G. schlechtendaliana REICHB. fil., G. matsumurana SCHLTR. and G. discolor KER- GAWL. The structures of 1 and 2 have been determined by NMR, MS spectroscopic and chemical means. Compound 1 was converted into its methyl ester form (5) during the purification step, when the lactone ring was cleaved by catalysis of silica gel with the CHCl₃-MeOH-H₂O as a solvent. On the other hand, 1 was obtained in a high yield by the same purification procedure without MeOH. Based on this fact, a simple and economic method for the purification of 1 was confirmed. Compounds 1 and 2 were found to have a hepatoprotective effect on liver injury induced by carbon tetrachloride in primary cultured rat hepatocytes.

Full text of DOI:10.1248/bpb.23.731

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