Regeneration of NADH and Hydrogenation of Ketones to Alcohols with the Combination of Hydrogenase and Alcohol Dehydrogenase

Article abstract of DOI:10.1246/cl.1987.2089

The regeneration of nicotinamide-adenine dinucleotide (reduced form, NADH) by the reduction of NAD with hydrogen gas was carried out in the presence of the hydrogenase from Alcaligenes eutrophus, and ketone hydrogenation was tried by the combination of the above system and alcohol dehydrogenase (ALDH).The ketones such as 2-butanone, 2-pentanone, and 2-hexanone were easily hydrogenated by hydrogen gas.After 65 h, the turnover number of NAD was 64 for 2-butanone showing a recurrence of NAD by hydrogen gas.