High fermentative production of L-threonine from acetate by a Brevibacterium flavum stabilized strain transformed with a recombinant plasmid carrying the Escherichia coli thr operon.

Article abstract of DOI:10.1271/bbb.57.1755

Decrease in L-threonine productivity caused not only by plasmid-free segregation but also by plasmid deletion was observed in a Brevibacterium flavum L-threonine producer when transformed with a recombinant plasmid carrying Escherichia coli thr operon. However, a recombinant strain, HT-16, was stabilized by the addition of trimethoprim (a selective marker on the vector) at concentration of 1000 micrograms/ml, which was rather higher than the minimum inhibitory one, into the stock culture medium. Strain HT-16 produced 64.4 g/liter of L-threonine, 30% higher than that of the host strain, after 92 h of cultivation in a small jar fermentor using acetic acid as a carbon source without trimethoprim.

Full text of DOI:10.1271/bbb.57.1755

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High Fermentative Production of L-Threonine from
Acetate by a Brevibacterium flavum Stabilized Strain
Transformed with a Recombinant Plasmid Carrying
the Escherichia coli thr Operon
Masaaki Ishidaa, Katsuaki Satoa, Ken-ichi Hashiguchia, Hisao Itoa, Hitoshi Eneia & Shigeru
Nakamoria
a Central Research Laboratories of Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku,
Kawasaki, Kanagawa 210, Japan
Published online: 12 Jun 2014.
To cite this article: Masaaki Ishida, Katsuaki Sato, Ken-ichi Hashiguchi, Hisao Ito, Hitoshi Enei & Shigeru Nakamori (1993)
High Fermentative Production of L-Threonine from Acetate by a Brevibacterium flavum Stabilized Strain Transformed
with a Recombinant Plasmid Carrying the Escherichia coli thr Operon, Bioscience, Biotechnology, and Biochemistry, 57:10,
1755-1756, DOI: 10.1271/bbb.57.1755
To link to this article: http://dx.doi.org/10.1271/bbb.57.1755
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