Exploiting Designed Oxidase-Peroxygenase Mutual Benefit System for Asymmetric Cascade Reactions

Article abstract of DOI:10.1021/jacs.9b01939

A unique P450 monooxygenase-peroxygenase mutual benefit system was designed as the core element in the construction of a biocatalytic cascade reaction sequence leading from 3-phenyl propionic acid to (R)-phenyl glycol. In this system, P450 monooxygenase (P450-BM3) and P450 peroxygenase (OleT_JE) not only function as catalysts for the crucial initial reactions, they also ensure an internal in situ H₂O₂ recycle mechanism that avoids its accumulation and thus prevents possible toxic effects. By directed evolution of P450-BM3 as the catalyst in the enantioselective epoxidation of the styrene-intermediate, formed from 3-phenyl propionic acid, and the epoxide hydrolase ANEH for final hydrolytic ring opening, (R)-phenyl glycol and 9 derivatives thereof were synthesized from the respective carboxylic acids in one-pot processes with high enantioselectivity.

Full text of DOI:10.1021/jacs.9b01939

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Communication
Exploiting Designed Oxidase-Peroxygenase Mutual
Benefit System for Asymmetric Cascade Reactions
Da Yu, Jian-bo Wang, and Manfred T. Reetz
J. Am. Chem. Soc., Just Accepted Manuscript • DOI: 10.1021/jacs.9b01939 • Publication Date (Web): 28 Mar 2019
Downloaded from http://pubs.acs.org on March 28, 2019
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