Table 1 HRMS (ESI-TOF) analysis of the synthesize NTPs
Measured (calcd.) [M ꢀ H]ꢀ m/z
Nucleoside triphosphate
Chemical formula
ATP
CTP
GTP
UTP
C
C
C
C
10
H
H
H
H
16
16
16
15
N
5
O
O
O
O
13
14
14
15
P
3
3
505.9891 (505.9885)
481.9781 (481.9772)
521.9828 (521.9834)
482.9615 (482.9612)
9
N
3
P
3
10
N
5
P
9
N
2
P
3
2
3
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Fig. 2 (A) DNA template and promoter sequences used in the
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32
transcripts were bodily labelled using [a- P]-ATP. These transcription
reactions (5.0 mL each) containing the DNA promoter (1.0 mM), the
DNA template (1.0 mM), NTPs (0.50 mM each) and RNA polymerase
1
1
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989, 23, 205–220.
(0.1 mL per microlitre reaction) were incubated at 37 1C for 2 h. Each
reaction was analyzed by 19% polyacrylamide gel electrophoresis.
Lane 1: containing DNA template/promoter and all NTPs, but no T7
RNA polymerase; Lane 2: containing DNA template/promoter, all
NTPs, and T7 RNA polymerase; Lane 3, 5, 7 and 9: containing DNA
template/promoter, T7 RNA polymerase, but missing ATP, CTP GTP
and UTP, respectively; Lane 4, 6, 8 and 10: ATP, CTP GTP and UTP
were added to the missed reactions, respectively. Lane 11: using all
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This work was financially supported by NSF (MCB-
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010, 75, 1501–16.
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824837), the Georgia Cancer Coalition (GCC) Distinguished
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8 M. A. Cheek, M. I. Dobrikov, C. K. Wennefors, Z. Xu,
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Cancer Clinicians and Scientists, and NIH (GM095086).
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144 Chem. Commun., 2011, 47, 8142–8144
This journal is c The Royal Society of Chemistry 2011