Photoaffinity labeling of PKC with a phorbol derivative: Importance of the 13-acyl group in phorbol ester-PKC interaction

Article abstract of DOI:10.1016/0040-4039(95)01864-E

We report the design and synthesis of a novel photoaffinity ligand, phorbol 12-(1-pyrenebutyrate) 13-diazoacetate (PPDA, 6). This photolabile phorbol derivative labeled with tritium was used for photocrosslinking experiments with a sample of PKC partially purified from rat brain, and specific binding of 6 to PKC was observed.