In Situ N-Phosphorylation of Oligopeptides for Fast Atom Bombardment Mass Spectrometry

Article abstract of DOI:10.1002/oms.1210270618

Positive ion fast atom bombardment mass spectrometry (FABMS) of in situ N-phosphorylated oligopeptides showed intense quasi-molecular ions together with the successive alkene loss fragment ions, which afford multiple checks of the unequivocal reality of the relative molecular mass of the tested samples.More interesting, in a novel cleavage pattern only the N-phosphoryl fragment ions gave intense peaks, the C-terminal series ions being suppressed.For each of the N-terminal ions, losses of alkenes also occur to provide multiple checks for the existence of these ions.The FABMS of the in situ N-phosphorylated oligopeptides might provide an easily accessible routine method for peptide sequencing.