Journal of the Chemical Society - Faraday Transactions p. 553 - 557 (1991)
Update date:2022-08-16
Topics:
Inoue, Hiroshi
Kubo, Yasuko
Yoneyama, Hiroshi
Photocatalytic fixation of CO2 in oxoglutaric acid to yield isocitric acid was investigated with the use of isocitrate dehydrogenase as a catalyst, methyl viologen as an electron mediator and cadmium sulphide as a photocatalyst.The rate of the isocitric acid production depended on the concentration of oxoglutaric acid and methyl viologen, and the rate equation to meet these findungs was derived.The Michaelis-Menten constant determined by using this equation (Km1 and Km2) was 23.9 mmol dm-3 for the substrate and 0.030 mmol dm-3 for the mediator, suggesting that the electron transfer between isocitrate dehydrogenase and the substrate is the rate-determining step.
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