CYCLODEXTRIN-FACILITATED BIOCONVERSION OF 17β-ESTRADIOL BY A PHENOLOXIDASE FROM MUCUNA PRURIENS CELL CULTURES

Article abstract of DOI:10.1016/0031-9422(90)80119-2

After complexation with β-cyclodextrin, the phenolic steroid 17β-estradiol could be ortho-hydroxylated into a catechol, mainly 4-hydroxyestradiol, by a phenoloxidase from in vitro grown cells of Mucuna pruriens.By complexation with β-cyclodextrin the solubility of the steroid increased from almost insoluble to 660μM.The bioconversion efficiency after 72 hr increased in the following order: freely suspended cells (0percent), immobilized cells (1percent), cell homogenate (6percent), phenoloxidase preparation (40percent).Mushroom tyrosinase converted 17β-estradiol, as a complex with β-cyclodextrin, solely into 2-hydroxyestradiol, with a maximal yield of 30percentafter 6-8 hr.Uncomplexed 17β-estradiol was not converted at all in any of these systems.