Synthesis of 2'-iodo- and 2'-bromo-ATP and GTP analogues as potential phasing tools for X-ray crystallography

Article abstract of DOI:10.1080/07328319908045600

Ara-adenosine (adenine 9-β-D-arabinofuranoside) and ara-guanosine (guanine 9-β-D-arabinofuranoside) are converted into 2' halogenated ATP and GTP analogues by triflation and subsequent inversion of configuration at C- 2'. For the commercially unavailable ara-guanosine a short synthesis starting from guanosine is presented. The nucleotide analogues could serve for the preparation of heavy atom derivatives of ATP- and GTP-binding proteins useful for protein crystal structure determination by MIR/MAD phasing.

Full text of DOI:10.1080/07328319908045600

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Synthesis of 2′-Iodo- and 2′-Bromo-ATP
and GTP Analogues as Potential Phasing
Tools for X-ray Crystallography
a
a
a
Mathias Gruen , Christian Becker , Andrea Beste , Christoph
b
a
a
Siethoff , Axel J. Scheidig & Roger S. Goody
a
Max-Planck-Institut für molekulare Physiologie , Rheinlanddamm
01, 44139, Dortmund, Germany
2
b
Institut für Spektrochemie and angewandte Spektroskopie ,
Bunsen-Kirchhoff-Strasse 21, 44139, Dortmund, Germany
Published online: 04 Oct 2006.
To cite this article: Mathias Gruen , Christian Becker , Andrea Beste , Christoph Siethoff , Axel J.
Scheidig & Roger S. Goody (1999) Synthesis of 2′-Iodo- and 2′-Bromo-ATP and GTP Analogues as
Potential Phasing Tools for X-ray Crystallography, Nucleosides and Nucleotides, 18:1, 137-151, DOI:
10.1080/07328319908045600
To link to this article: http://dx.doi.org/10.1080/07328319908045600
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