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RSC Advances
DOI: 10.1039/C4RA04552A
4.975
5.34
8.2
99.5
106.8
102.5
103.6
2.09
5.74
1.27
2.12
15 S. K. Ghosh and T. Pal, Chem. Rev., 2007, 107, 4797ꢀ4862.
16 H. Li and L. J. Rothberg, J. Am. Chem. Soc., 2004, 126, 10958ꢀ
Volunteer Ⅰ
5
8
Volunteer Ⅱ
Volunteer Ⅰ
Volunteer Ⅱ
60
10961.
17 H. Wei, B. Li, J. Li, E. Wang and S. Dong, Chem. Commun., 2007,
3735ꢀ3737.
18 J. Wang, Y. F. Li, C. Z. Huang and T. Wu, Anal. Chim. Acta., 2008,
8.29
626, 37ꢀ43.
65 19 Q. Xiao, F. Shang, X. Xu, Q. Li, C. Lu and J.M. Lin, Biosensors and
Bioelectronics, 2011, 30, 211ꢀ215.
20 L. Li and B. Li, Analyst, 2009, 134, 1361ꢀ1365.
21 G. Patel and S. Menon, Chem. Commun., 2009, 3563ꢀ3565.
22 K. Ai, Y. Liu and L. Lu, J. Am. Chem. Soc., 2009, 131, 9496ꢀ9497.
70 23 G .Frens, Nature physical science, 1973, 241, 20ꢀ22.
24 P. K. Sudeep, S. T. Shibu Joseph and K. George Thomas, J. Am.
Chem. Soc., 2005, 127, 6516ꢀ6517.
In summary, we have developed a novel and simple strategy
based on unmodified AuNPs for the colorimetric detection of
cystine. Cystine is effectively reduced by nontoxic ascorbic acid
to cysteine, inducing the aggregation of unmodified AuNPs
through the efficient electrostatic interaction between cysteineꢀ
bonded AuNPs. The color detection limit of the proposed method
is down to 1 ꢁM with the naked eye, which is more sensitive than
previously reported colorimetric methods. The practicability of
this method was validated through analyses of real urine samples.
5
25 B. Lux, P. May, Urol. Int., 1983, 38, 91ꢀ94.
26 H. Birwe and A. Hesse, Clinica Chimica Acta, 1991, 199, 33ꢀ42.
75 27 K.Kusmierek, R.Glowacki, and E.Bald, Anal Bioanal Chem, 2006,
385, 855ꢀ860.
10 Compared with the existing methods for measurement of cystine
in human urine, the colorimetric assay method demonstrated here
is advantageous in terms of its simplicity and nontoxicity, and is
thus highly anticipated to assist in the diagnosis of human
cystinuria.
28 J. L. Burguera, M. R. Brunetto, Y. Contreras, M. Burguera, M.
Gallignani and P. Carrero, Talanta, 1996, 43, 839ꢀ850.
15
The authors acknowledge the geological survey project of China
Geological Survey (12120113015300) and the special funding
support from the Fundamental Research Funds for the Central
Universities (CUG090108).
20 Notes and references
a Nano-Mineral Materials and Application Engineering Research Center
of Ministry of Education, Faculty of Materials Science and Chemistry,
China University of Geosciences, Wuhan 430074, P. R. China. E-mail:
25 b Division of Nanomaterials and Chemistry, Hefei National Laboratory
for Physical Sciences at Microscale, Department of Chemistry, University
of Science and Technology of China, Hefei 230026, P. R. China.
† Electronic Supplementary Information (ESI) available: experimental
section and variables optimization. See DOI: 10.1039/b000000x/
30 ‡ Footnotes should appear here. These might include comments relevant
to but not central to the matter under discussion, limited experimental and
spectral data, and crystallographic data.
1
M. J. Calonge, P. Gasparini, J. Chillarón , M. Chillón, M. Gallucci, F.
Rousaud, L. Zelante, X. Testar, B. Dallapiccola, F. Di Silverio, P.
Barceló, X. Estivill, A. Zorzano, V. Nunes and M. Palacín, Nature
Genetics, 1994, 6, 420ꢀ425.
35
40
45
2
S. R. Karl and C. M. James, Saudi J Kidney Dis Transplant, 2003, 14
(1): 351ꢀ357.
R. W. Virtue and H. B. Lewis, J. Biol. Chem., 1934, 104, 415.
Y. Wang, X. J. Kang and W. H. Ge, Chromatographia, 2007, 65,
527ꢀ532.
P. Mikus, P. Kubacak, I. Valaskova and E. Havaranek, Pharmazie,
2003, 58, 111ꢀ113.
G. Li, J. Yang, X. Zheng, M. Meng and J. Cao, Microchim Acta,
2010, 168, 277ꢀ282.
3
4
5
6
7
M. S. Damle, L. A. A. Newton, M. M. Villalba, R. Leslie and J.
Davis, Electroanalysis, 2010, 21, 2491ꢀ2495.
J. Chrastil, Analyst, 1989, 114, 1133ꢀ1136.
8
9
J. Chrastil, Analyst, 1990, 115, 1383ꢀ1384.
50 10 A. A. Ensafi, B. Rezaei and S. Nouroozi, J. Braz. Chem. Soc., 2009,
2, 288ꢀ293.
11 Y. Morioka and K. Kobayashi, Biol. Pharm. Bull., 1997, 20, 825ꢀ
827.
12 I. W. Grote, J. Biol. Chem., 1931, 93, 25ꢀ30.
55 13 K. Shinohara, J. Biol., Chem., 1935, 109, 665ꢀ679.
14 M. Hu, J. Chen, Z. Li, L. Au, G.V. Hartland, X. Li, M. Marquez and
Y. Xia, Chem. Soc. Rev., 2006, 35, 1084ꢀ1094.
4
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