Studies on enzymatic activity of alkaline phosphatase
Interestingly, the degradation of AMP to adenosine was
demonstrated to be distinctively slower, but mainly in very
alkaline medium. Nevertheless, a reduced degradation rate
of AMP substrate compared to ATP or ADP degradation
rates was also observed at physiological pH, although the
difference was less pronounced. Physiological consequences
in the context of this disparity that may be related to a more
outstanding role for AMP in the context of inflammation or
pH regulatory processes in the intestinal tract have to be
enlightened in the future.
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Acknowledgements
The authors would like to thank Vital Solutions GmbH
(
(
(
Langenfeld, Germany) and Amino Up Chemicals Co., Ltd
Sapporo, Japan) for financial support. We also thank Knauer
Wissenschaftliche Gerätebau Dr. Ing. Herbert Knauer, Berlin,
Germany) for the loan of the single-quadrupole mass
spectrometer and Stefan Bieber for his dedication and his
excellent work.
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