INVESTIGATIONS IN THE FIELD OF ACYL GROUP CARRIERS. XVI. HYDROLYTIC STABILITY AND ENZYMATIC DEPHOSPHORYLATION OF D-PANTOTHENIC ACID 4'-PHOSPHATE

Article abstract of DOI:10.1007/BF01185268

Samples of D-pantothenic acid 4'-phosphate (I) have been investigated by IR spectroscopy in dimethyl sulfoxide and deuterated water and in the form of a thin film without a solvent.The vibrations of the carbonyl and amide groupings in (I) as compared with pantothenic acid (II) are found at higher frequencies in all media, which indicate the greater strength of these bonds.It is assumed that the resistance of (I) to hydrolysis as compared with (II) is due to the inclusion of the phosphate in a macrocycle by the formation of hydrogen bonds between the phosphate, hydroxy, amide, and carboxy groups of the molecule.The complete dephosphorylation of (I) has been achieved in concentrations of 0.0075 and 0.005 mM with the aid of a dialyzed preparation of orthophosphate monoester phosphohydrolase (E.C. 3.1.3.1).