Chemical Science
Edge Article
and requirements are very well satised by the new 20-OCF3 14 C. Li, G.-F. Wang, Y. Wang, R. Creager-Allen, E. A. Lutz,
label as shown in this study; it therefore has potential to
H. Scronce, K. M. Slade, R. A. S. Ruf, R. A. Mehl and
advance to the most broadly applicable uorine label in NMR
G. J. Pielak, J. Am. Chem. Soc., 2010, 132, 321.
spectroscopy of nucleic acids. The 20-OCF3 group possesses 15 E. Malek-Adamian, D. C. Guenther, S. Matsuda, S. Martınez-
´
pronounced sensitivity and exhibits large chemical shi
dispersion so that it is possible to distinguish distinct base
sequences in double helical regions. Moreover, the ribose 20
position guarantees principal synthetic accessibility to all of the
four nucleosides. Additionally, the size of the OCF3 label
Montero, I. Zlatev, J. Harp, M. Burai Patrascu, D. J. Foster,
J. Fakhoury, L. Perkins, N. Moitessier, R. M. Manoharan,
N. Taneja, A. Bisbe, K. Charisse, M. Maier, K. G. Rajeev,
M. Egli, M. Manoharan and M. Damha, J. Am. Chem. Soc.,
2017, 139, 14542.
matches the naturally occurring and widespread 20-OCH3 16 M. Hennig, L. G. Scott, E. Sperling, W. Bermel and
group. For the investigation of naturally occurring 20-OCH3
J. R. Williamson, J. Am. Chem. Soc., 2007, 129, 14911.
modication patterns in biologically meaningful settings, the 17 B. Puffer, C. Kreutz, U. Rieder, M. O. Ebert, R. Konrat and
20-OCF3 group will be an ideal candidate.
R. Micura, Nucleic Acids Res., 2009, 37, 7728.
18 M. Hennig, M. L. Munzarova, W. Bermel, L. G. Scott,
V. Sklenar and J. R. Williamson, J. Am. Chem. Soc., 2006,
128, 5851.
Conflicts of interest
19 L. G. Scott and M. Hennig, Methods Enzymol., 2016, 566, 59.
20 M. Olejniczak, Z. Gdaniec, A. Fischer, T. Grabarkiewicz,
L. Bielecki and R. W. Adamiak, Nucleic Acids Res., 2002, 30,
4241.
There are no conicts to declare.
Acknowledgements
21 B. Luy and J. P. Merino, J. Biomol. NMR, 2001, 20, 39.
We thank Ulrike Rieder (Innsbruck) for initial 19F-NMR
measurements of the Fn preQ1 riboswitch, Martin Tollinger
(Innsbruck) for advice in data tting, and Vincent Olieric for
help during data collection at SLS, Villigen. This work was
supported by the Austrian Science Fund FWF (P27947, P31691,
F8011-B to R. M; P32773 to C. K.), the Austrian Research
Promotion Agency FFG [West Austrian BioNMR 858017], and
the Wiener Wissenschas-, Forschungs-und Technologiefonds
(WWTF LS17-003).
¨
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