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concentration, the proportion of lysosomes obviously
decreased, indicating evident inhibition of autophagy. Notably,
the cytoplasm emission obviously enhanced with the elevated
CQ concentration, indicating that the increase of cytoplasm pH
is accompanied with autophagy inhibition.
Conflicts of interest
There are no conflicts to declare.
DOI: 10.1039/C9CC03679B
Acknowledgement
For financial support, we thank the National Natural Science
Foundation of China (NSFC) (21472067, 21672083, 21877048,
2
1804052), the Taishan Scholar Foundation (TS201511041), the
Natural Science Foundation of Shandong Province
(
(
ZR2018BB058), and the startup funds of University of Jinan
309-10004, 160100331).
Notes and references
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Figure 4. The fluorescence images of HepG2 cells pre-stained with 5 μM Cyto-Lyso for 30
min (a) then incubated in PBS containing different amount of CQ for 2 h. Bar = 20 μm.
5
6
NH
4
3
Cl has been reported to cause the increase of intracellular
2
pH, and the effect of NH
4
Cl on autophagy was also studied in
(
4), 664-673.
Figure S8. Compared with the cells starved for 2 h, addition of
0 μM NH Cl would not bring obvious effect on autophagy.
However, the cytoplasm pH is apparently increased compared
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In summary, a dual-site fluorescent probe (Cyto-Lyso) for the
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4
starvation induced autophagy. The inhibition effect of NH Cl
2
2
and CQ on autophagy have been also investigated with the
probe. The probe can serve as a valid tool for the assessment of
pH changes in cytoplasm and lysosomes simultaneously, and
facilitate the fundamental researches on autophagy and 23 Hagag, N.; Lacal, J. C.; Graber, M.; Aaronson, S.; Viola, M. V.,
relative areas.
Mol. Cell. Biol. 1987, 7 (5), 1984-1988.
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