ChemComm
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COMMUNICATION
DOI: 10.1039/C5CC00644A
significantly less reduction, with mean values of 38%, 39% and
This research was financially supported by a Marie Curie IEF
33.6%, respectively. When the effect of the DOXꢀloaded NGs on the (Project 302717), the ThermoNanogele NanoMatFutur award
morphology and proliferation of cancer cells was analysed in real (13N12561), and the focus area Nanoscale of the FU Berlin.
time, the NGs with all monomers showed again the most similar
profile to that of free DOX (Fig. S7, ESI). The NGs lacking any of
Notes and references
a Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse
3, 14195 Berlin, Germany. Eꢀmail: marcelo.calderon@fuꢀberlin.de
the responsive moieties were less effective and presented very
dissimilar profiles, suggesting that the combination of the different
stimuli in the multiresponsive NGs may have a synergistic effect.
†
Electronic Supplementary Information (ESI) available: Experimental
section and supplemental figures. See DOI: 10.1039/c000000x/
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Conclusions
We have developed a simple, reproducible, oneꢀpot synthetic
approach that allows the preparation of DOXꢀloaded multiresponsive
NGs based on biocompatible hPG. This strategy combines free
radical polymerization, nanoprecipitation, and highꢀloading drug
encapsulation in a single step. The method here described could
be applied for the in situ encapsulation of other drugs or
bioactive molecules into NGs prepared by polymerization
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4 | J. Name., 2012, 00, 1-3
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