Carbohydrate Research p. 123 - 132 (1985)
Update date:2022-08-24
Topics:
Defaye, Jacques
Driguez, Hugues
John, Michael
Schmidt, Juergen
Ohleyer, Eric
The D-xylan degrading enzymes of Trichoderma lignorum can be induced by several oligo- and poly-saccharides.The induction is optimal when L-arabino-D-xylan is used as carbon source.The enzymes were also produced by cells grown on glycerol in the presence of 4-thioxylobiose (4-S-β-D-xylopyranosyl-4-thio-D-xylopyranose)and methyl β-D-xylopyranoside.These inducers were not taken up by the cells.The extent of induction was, however, lower than with xylobiose.Thioxylobiose was obtained in an overall yield of 55-60percent from the reaction of 1,2,3-tri-O-benzoyl-4-O-trifluoromethylsulfonyl-β-L-arabinopyranose with the sodium salt of 1-thio-β-D-xylopyranose in N',N'',N'''-hexamethylphosphoramide, followed by O-deacylation of the resulting 4-thiodisaccharide.
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