Characterization of hepatic flavin monooxygenase from the marine teleost turbot (Scophthalmus maximus L.)

Article abstract of DOI:10.3109/00498259509061838

1. The presence and properties of flavin monooxygenase (FMO) in liver of the marine teleost, turbot (Scophthalmus maximus) were examined in relation to organic xenobiotic metabolism and osmoregulation. 2. Hepatic microsomes of sexually mature fish contained NADPH-dependent FMO as evidenced by the conversion of N,N-dimethylaniline (DMA) to DMA-N-oxide, and immunorecognition of single bands (approximate apparent molecular weight of 55 kDa) by antibodies to mammalian FMO 1 and FMO 2. Additionally, Northern analysis using a full-length cDNA probe to mammalian FMO 1 revealed a single hybridizing band of approximately 2.5 kb. 3. No significant differences were seen between male and female turbot FMO with respect to DMA N-oxidase activity, levels of immunoreactive protein (with anti-FMO 1 or anti-FMO 2) and gene expression (hybridizing mRNA). 4. Hepatic microsomal DMA N-oxidase activity was inhibited by methimazole (an FMO substrate) and trimethylamine (TMA), but not by piperonyl butoxide (a P450 inhibitor). Inhibition by TMA is indicative of a role for FMO in osmoregulation, catalysing the conversion of TMA to TMA N-oxide. DMA N-oxidase activity was optimal at pH 8.8 and 25°C, and displayed Michaelis-Menten kinetics with respect to DMA (apparent Km = 88 μM).

Full text of DOI:10.3109/00498259509061838

Xenobiotica
the fate of foreign compounds in biological systems
ISSN: 0049-8254 (Print) 1366-5928 (Online) Journal homepage: http://www.tandfonline.com/loi/ixen20
Characterization of hepatic flavin monooxygenase
from the marine teleost turbot (Scophthalmus
maximus L.)
L. D. Peters, D. R. Livingstone, S. Shenin-Johnson, R. N. Hines & D. Schlenk
To cite this article: L. D. Peters, D. R. Livingstone, S. Shenin-Johnson, R. N. Hines & D. Schlenk
(1995) Characterization of hepatic flavin monooxygenase from the marine teleost turbot
(Scophthalmus maximus L.), Xenobiotica, 25:2, 121-131
To link to this article: http://dx.doi.org/10.3109/00498259509061838
Published online: 27 Aug 2009.
Submit your article to this journal
Article views: 10
View related articles
Citing articles: 1 View citing articles
Full Terms & Conditions of access and use can be found at
http://www.tandfonline.com/action/journalInformation?journalCode=ixen20
Download by: [Imperial College London Library]
Date: 07 November 2015, At: 23:01