Practical Preparation of K-252a from a Fermentation Solution

Article abstract of DOI:10.1271/bbb.62.1627

We developed a practical preparation procedure for K-252a by methylating K-252b on an industrial scale. The water-insoluble K-252a, which was present in the cell mass, was converted to the water-soluble K-252b Na salt in an alkaline solution. The obtained K-252b was methylated with dimethylsulfate in the presence of potassium carbonate in dimethylacetamide. We have already used this method to manufacture 90 kg of K-252b from the fermentation broth, and regenerated 65 kg of K-252a from K-252b.

Full text of DOI:10.1271/bbb.62.1627

Bioscience, Biotechnology, and Biochemistry
ISSN: 0916-8451 (Print) 1347-6947 (Online) Journal homepage: http://www.tandfonline.com/loi/tbbb20
Practical Preparation of K-252a from a
Fermentation Solution
Mitsutaka KINO, Kenzo SHONO, Tetsuo NISHIMURA & Satoru NAGAMURA
To cite this article: Mitsutaka KINO, Kenzo SHONO, Tetsuo NISHIMURA & Satoru NAGAMURA
(1998) Practical Preparation of K-252a from a Fermentation Solution, Bioscience,
Biotechnology, and Biochemistry, 62:8, 1627-1629, DOI: 10.1271/bbb.62.1627
To link to this article: http://dx.doi.org/10.1271/bbb.62.1627
Published online: 22 May 2014.
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Date: 06 June 2016, At: 09:08