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Name	Thiazolyl Blue	EINECS	206-069-5
CAS No.	298-93-1	Density	1.4886 (estimate)
PSA	75.72000	LogP	0.28830
Solubility	slightly soluble in water	Melting Point	195 °C (dec.)(lit.)
Formula	C₁₈H₁₆BrN₅S	Boiling Point	N/A
Molecular Weight	414.329	Flash Point	N/A
Transport Information	N/A	Appearance	yellow crystalline powder
Safety	22-24/25-53-45	Risk Codes	46
Molecular Structure		Hazard Symbols	Xi, T
Synonyms	2H-Tetrazolium,2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (8CI,9CI);3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (6CI,7CI);(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide);2,5-Diphenyl-3-(4,5-dimethylthiazol-2-yl)-2H-tetrazolium bromide;2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazolium bromide;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide;3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide;MMTTetrazolium;MTT;MTT tetrazolium;NSC 60102;Thiazolyl blue (Sigma);Thiazolyl blue tetrazolium bromide;

Thiazolyl Blue Synthetic route

N-<4,5-dimethyl-thiazol-2-yl>-3,N'''-diphenyl-formazan: N-<4,5-dimethyl-thiazol-2-yl>-3,N'''-diphenyl-formazan

: 298-93-1
thiazolyl blue

Conditions

Conditions	Yield
With N-Bromosuccinimide; ethyl acetate

: 7631-95-0
sodium molybdate dihydrate

: 3316-09-4
1,2-dihydroxy-4-nitrobenzene

: 298-93-1
thiazolyl blue

: [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium][MoO2(4-nitrocatechol(-2H))2]

Conditions

Conditions	Yield
In water mixing of aq. solns. of Na2MoO4*2H2O, 4-nitrocatechol and 3-(4,5-dimethylthiaozol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide in acidic medium (pH2.8 - 4.0); pptn., filtration, washing with cold water, drying at 75-80°C;

: 298-93-1
thiazolyl blue

: 23305-68-2
1-(4,5-dimethyl-2-thiazoyl)-3,5-diphenylformazan

Conditions

Conditions	Yield
With dehydrogenase
With succinate dehydrogenase at 37℃; for 4h;

: iron(III) ammonium sulfate dodecahydrate

: 3316-09-4
1,2-dihydroxy-4-nitrobenzene

: 298-93-1
thiazolyl blue

: Fe(3+)*3C6H3NO4(2-)*3C18H16N5S(1+)

Conditions

Conditions	Yield
With sulfuric acid In water

: ammonium metavanadate

: 298-93-1
thiazolyl blue

: 37422-56-3
5-methyl-4-(2-thiazolylazo)resorcinol

: 2O2V(1+)*2C10H7N3O2S(2-)*2C18H16N5S(1+)

Conditions

Conditions	Yield
In chloroform; water

: 298-93-1
thiazolyl blue

: (E,Z)-5-(4,5-dimethylthiazol-2-yl)-1,3-diphenylformazan

Conditions

Conditions	Yield
With mitochondrial reductase Enzymatic reaction;

: 298-93-1
thiazolyl blue

: MTT formazan metabolite

Conditions

Conditions	Yield
With A549 cells for 1h; Enzymatic reaction;
With mitochondrial dehydrogenase Enzymatic reaction;

Thiazolyl Blue Chemical Properties

IUPAC Name: Thiazolyl blue
The MF of Thiazolyl blue (298-93-1) is C₁₈H₁₈BrN₅S.

The MW of Thiazolyl blue (298-93-1) is 416.34.
Synonyms of Thiazolyl blue (298-93-1): 3-(4,5-Dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide ; 2-(4,5-Dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-2H-tetrazol-3-ium bromide ; 2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide
Product Categories: Tetrazolium Salts;Tetrazolium Salts & Formazans;Chemistry
Form: yellow crystalline powder
EINECS: 206-069-5
Melting Point: 195 °C
Storage temp: 2-8°C
Solubility in methanol: 5 mg/mL, clear
Sensitive: Light Sensitive
BRN: 3825277

Thiazolyl Blue Toxicity Data With Reference

1.		mmo-sat 5 µg/plate		MUREAV Mutation Research. 68 (1979),107.
2.		mmo-esc 5 µg/plate		MUREAV Mutation Research. 68 (1979),107.

Thiazolyl Blue Safety Profile

Mutation data reported. When heated to decomposition it emits very toxic fumes of NO_x and SO_x. See also BROMIDES.
Safety information of Thiazolyl blue (298-93-1):
Hazard Codes Xi,T
Risk Statements
46 May cause heritable genetic damage
Safety Statements
22 Do not breathe dust
24/25 Avoid contact with skin and eyes
53 Avoid exposure - obtain special instruction before use
45 In case of accident or if you feel unwell, seek medical advice immediately (show label where possible)
WGK Germany 3
RTECS XF8060000
F 3-8-10
HS Code 29341000

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