Ligand Binding Assay

A technique to measure the interaction between a receptor and its ligand.

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Synthesis NMR spectroscopy Heterocyclic compounds Selectivity Molecular docking IC50 Pharmacophore Potency Enantiomers Structure-activity relationships (SAR) X-ray crystallography In Vivo Testing Lead Optimization Design Structure-Activity Relationship (SAR) Computational Chemistry High-Throughput Screening (HTS) Dose-Response Curve Structure-Based Drug Design (SBDD) Toxicity Profile In vitro assay In vivo assay Negative allosteric modulators (NAMs) Receptor antagonist Bioisostere Radioligand Binding Assay Metabotropic glutamate receptor 5 (mGluR5) Allosteric site Selectivity Profile Ki (Inhibition constant) IC?? Lipinski’s Rule of Five EC50 ADME (Absorption, Distribution, Metabolism, Excretion)

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