The reaction conditions should be optimized to ensure high yield and site-specificity. Characterization techniques such as mass spectrometry, NMR spectroscopy, and HPLC are used to confirm the successful arylation of Trp residues in the peptide.
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(S)-N-acetyl-2-(3,4-dimethylphenyl)tryptophan methyl ester
5-Morpholinothiophene-2-carboxylic acid
