A coupling agent, such as dicyclohexylcarbodiimide (DCC), is used to react the primary amine with a suitable thiol compound, such as 3-mercaptopropionic acid. This forms the peptide bond.
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3-chloro-2-methylpropionic acid
Z-LEU-GLY-OH
L-Leucine, N-[(phenylmethoxy)carbonyl]-L-alanyl-L-alanyl-
