To study the equilibrium between fluoride ions and the thiourea derivative, you can perform a titration experiment. You start with a solution of the thiourea derivative containing the fluorophore and gradually add fluoride ions. As fluoride ions bind to the N-H groups in the thiourea derivative, the fluorescence intensity will decrease as the equilibrium shifts towards the formation of hydrogen bonds.
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2-[(4,4-dimethyl-2,6-dioxo-cyclohexyl)-(4-methoxyphenyl)methyl]-5,5-dimethyl-cyclohexane-1,3-dione
N-(4-bromobenzyl)-N'-(tert-butoxycarbonyl)thiourea
