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2635-84-9

Basic Information
CAS No.: 2635-84-9
Name: Butanoic acid,4-nitrophenyl ester
Molecular Structure:
Molecular Structure of 2635-84-9 (Butanoic acid,4-nitrophenyl ester)
Formula: C10H11NO4
Molecular Weight: 209.2
Synonyms: 4-Nitrophenyl butanoate;Butanoic acid p-nitrophenyl ester;(4-Nitrophenyl) butanoate;4-Nitrophenyl butyrate;Butanoic acid,4-nitrophenyl ester (9CI);Butyric acid,p-nitrophenyl ester;Butyric acid, p-nitrophenyl ester (8CI);p-Nitrophenol butyrate;p-Nitrophenyl butyrate;
EINECS: 258-258-3
Density: 1.216 g/cm3
Melting Point:
Boiling Point: 323.2 °C at 760 mmHg
Flash Point: 144.8 °C
Solubility: Chloroform: 0.1 g/mL, clear, faintly yellow
Appearance: Clear light brown liquid
Hazard Symbols: IrritantXi
Risk Codes: 43
Safety: 36/37
Transport Information:
PSA: 72.12000
LogP: 2.82350
Synthetic route
100-02-7

4-nitro-phenol

141-75-3

butyryl chloride

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
With pyridine In dichloromethane at 0℃; for 3h;99%
With iodine; magnesium; benzene
With magnesium; benzene
100-02-7

4-nitro-phenol

107-92-6

butyric acid

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
With dicyclohexyl-carbodiimide In ethyl acetate at 20℃; Inert atmosphere;90%
With dmap; dicyclohexyl-carbodiimide In dimethyl sulfoxide at 20℃; for 8h;81.5%
Stage #1: butyric acid With dmap; dicyclohexyl-carbodiimide In dichloromethane at 0℃; for 0.5h;
Stage #2: 4-nitro-phenol In dichloromethane at 0℃; for 6h;
70%
14617-88-0

(E)-4-nitrophenyl but-2-enoate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
With diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate; 3,4-dihydro-2H-benzo[4,5]thiazolo[3,2-a]pyrimidine In toluene at 110℃; for 1h;59%
With tri-n-butyl-tin hydride; magnesium bromide In dichloromethane at 0℃; for 27h;40%
With tri-n-butyl-tin hydride; magnesium bromide In dichloromethane at 0℃; for 27h;40%
106-31-0

butanoic acid anhydride

100-02-7

4-nitro-phenol

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
In 1,4-dioxane; pyridine for 2h; Ambient temperature;52%
100-02-7

4-nitro-phenol

71-36-3

butan-1-ol

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
With [RhCl2(p-cymene)]2; oxygen; caesium carbonate; 1,3-bis[(2,6-diisopropyl)phenyl]imidazolinium chloride In 5,5-dimethyl-1,3-cyclohexadiene at 130℃; for 24h;50%

Butyric acid 4-nitro-phenyl ester; compound with 1-methyl-1H-imidazole

A

616-47-7

1-methyl-1H-imidazole

B

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
With 2-(cyclohexylamino)ethanesulfonic acid In acetonitrile at 27℃; Equilibrium constant;
100-02-7

4-nitro-phenol

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 29 steps
1: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
2: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
3: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
4: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
5: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
6: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
7: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
8: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
9: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
10: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
11: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
12: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
13: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
14: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
15: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
16: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
17: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
18: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
19: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
20: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
21: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
22: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
23: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
24: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
25: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
26: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
27: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
28: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
29: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 11 steps
1: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
2: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
3: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
4: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
5: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
6: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
7: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
8: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
9: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
10: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
11: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 11 steps
1: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
2: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
3: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
4: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
5: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
6: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
7: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
8: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
9: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
10: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
11: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
4195-18-0

4-nitrophenyl 4-methylpentanoate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 22 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 18 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 20 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
1956-09-8

4-nitrophenyl decanoate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 26 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
23: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
24: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
25: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
26: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 22 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 24 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
23: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
24: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
13551-17-2

4-nitrophenyl ester cyclohexanecarboxylic acid

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 10 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 10 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 10 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
17895-71-5

p-nitrophenyl hydrocinnamate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 4 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 2 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 4 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
14617-86-8

4-nitrophenyl stearate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 28 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
23: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
24: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
25: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
26: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
27: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
28: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 24 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
23: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
24: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 26 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
23: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
24: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
25: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
26: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
959-22-8

p-nitrophenylbenzoate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 6 steps
1: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 4 steps
1: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 4 steps
1: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 6 steps
1: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
42721-91-5

4-nitrophenyl cyclopentanecarboxylate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 12 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 10 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 10 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
26050-99-7

4-nitrophenyl cyclopropanecarboxylate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 16 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 12 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 14 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
72289-61-3

phenyl-2 butyrate de p-nitrophenyle

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 8 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 4 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 6 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme

4-nitrophenyl 2-methylpentanoate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 18 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 14 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 16 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme

4-nitrophenyl 3-methylpentanoate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 20 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 16 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 18 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
830-03-5

4-nitrophenol acetate

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 6 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 2 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 4 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 4 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 6 steps
1: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
321865-45-6

cyclobutanecarboxylic acid 4-nitrophenyl ester

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 14 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 10 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 12 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
104013-15-2

2-phenylpropionic acid p-nitrophenyl ester

2635-84-9

p-nitrophenyl butyrate

Conditions
ConditionsYield
Multi-step reaction with 24 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Bacillus subtilis 168 PnbA esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
23: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
24: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 20 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
Multi-step reaction with 22 steps
1: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
2: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
3: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
4: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
5: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
6: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
7: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
8: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
9: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
10: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
11: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
12: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
13: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
14: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
15: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
16: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
17: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
18: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
19: Candida antarctica lipase B / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
20: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
21: Bacillus subtilis 168 SrfAD esterase / dimethyl sulfoxide / 0.05 h / 30 °C / pH 7 / aq. phosphate buffer; Enzymatic reaction
22: dmap; dicyclohexyl-carbodiimide / dimethyl sulfoxide / 8 h / 20 °C
View Scheme
2635-84-9

p-nitrophenyl butyrate

55536-08-8

ethyl 4-aminophenyl acetate

Conditions
ConditionsYield
With hydrogen; platinum(IV) oxide In ethanol93%
With palladium on activated charcoal; hydrogen In methanol at 20℃; for 16h;
2635-84-9

p-nitrophenyl butyrate

53917-01-4

1-(4-methoxyphenyl)butan-2-one

612825-12-4

4-(4'-methoxyphenyl)-octane-3,5-dione

Conditions
ConditionsYield
With dibenzo-18-crown-6; sodium hydride In tetrahydrofuran Heating;89%
2635-84-9

p-nitrophenyl butyrate

122-84-9

4-methoxybenzyl methyl ketone

498572-42-2

2-(4'-methoxyphenyl)-heptane-2,4-dione

Conditions
ConditionsYield
With dibenzo-18-crown-6; sodium hydride In tetrahydrofuran Heating;88%
2635-84-9

p-nitrophenyl butyrate

151412-40-7, 176298-42-3

1,3-Calix[5]crown-5

C67H90O9

Conditions
ConditionsYield
With (Ba)ClO4; hydrogen bromide; N-ethyl-N,N-diisopropylamine In methanol; acetonitrile at 25℃; for 3h;84%

ethyl (2S,3R,4R)-2-azido-4-fluoro-3-hydroxyoctadecanoate

2635-84-9

p-nitrophenyl butyrate

1259040-43-1

ethyl 2-(butanoylamino)-4-fluoro-3-hydroxyoctadecanoate

Conditions
ConditionsYield
With triphenylphosphine In tetrahydrofuran; water at 20℃; Staudinger ligation reaction; Inert atmosphere;82%
36160-02-8

N-p-Toluenesulfonyliminoisoquinolinium Ylide

2635-84-9

p-nitrophenyl butyrate

(1S,10bS)-1-ethyl-3-tosyl-1,10b-dihydropyrazolo[5,1-a]-isoquinolin-2(3H)-one

Conditions
ConditionsYield
With (5aS,10bR)-5a,10b-dihydro-2-(2,4,6-trimethylphenyl)-4H,6H-indeno[2,1-b]-1,2,4-triazolo[4,3-d]-1,4-oxazinium chloride; caesium carbonate In ethyl acetate at 20℃; Inert atmosphere; Schlenk technique; enantioselective reaction;75%
2635-84-9

p-nitrophenyl butyrate

1774-47-6

trimethylsulfoxonium iodide

1-[dimethyl(oxo)-λ6-sulfaneylidene]pentan-2-one

Conditions
ConditionsYield
Stage #1: trimethylsulfoxonium iodide With potassium tert-butylate In tetrahydrofuran for 2h; Inert atmosphere; Reflux;
Stage #2: p-nitrophenyl butyrate In tetrahydrofuran at 0 - 20℃; for 3h; Inert atmosphere;
75%
2635-84-9

p-nitrophenyl butyrate

309919-21-9

3-(6'-dodecylpyridin-2'-yl)-2-aminopropane-1,3-diol

(2S,3S)-3-(6'-dodecylpyridin-2'-yl)-2-butanoylamidopropane-1,3-diol

Conditions
ConditionsYield
In tetrahydrofuran at 20℃; for 48h; Acylation;70%

ethyl (2S,3R,4S)-2-azido-4-fluoro-3-hydroxyoctadecanoate

2635-84-9

p-nitrophenyl butyrate

1259040-45-3

ethyl 2-(butanoylamino)-4-fluoro-3-hydroxyoctadecanoate

Conditions
ConditionsYield
With triphenylphosphine In tetrahydrofuran; water at 20℃; Staudinger ligation reaction; Inert atmosphere;67%
2635-84-9

p-nitrophenyl butyrate

857005-50-6

N-Ts-1-(2-aminophenyl)-3-phenylprop-2-en-1-one

1644448-44-1

C26H23NO3S

Conditions
ConditionsYield
With 6,7-dihydro-2-phenyl-5H-pyrrolo-1,2,4-triazolium tetrafluoroborate; 1,8-diazabicyclo[5.4.0]undec-7-ene In toluene at 20℃; for 48h; Molecular sieve;63%

ethyl (2R,3S,4S)-2-azido-4-fluoro-3-hydroxyoctadecanoate

2635-84-9

p-nitrophenyl butyrate

1259040-44-2

ethyl 2-(butanoylamino)-4-fluoro-3-hydroxyoctadecanoate

Conditions
ConditionsYield
With triphenylphosphine In tetrahydrofuran; water at 20℃; Staudinger ligation reaction; Inert atmosphere;60%

ethyl (2R,3S,4R)-2-azido-4-fluoro-3-hydroxyoctadecanoate

2635-84-9

p-nitrophenyl butyrate

1259040-46-4

ethyl 2-(butanoylamino)-4-fluoro-3-hydroxyoctadecanoate

Conditions
ConditionsYield
With triphenylphosphine In tetrahydrofuran; water at 20℃; Staudinger ligation reaction; Inert atmosphere;59%
2635-84-9

p-nitrophenyl butyrate

11027-63-7

agnusoside

1105707-85-4

6-O-butanoyl-agnuside

Conditions
ConditionsYield
With Candida antarctica lipase B In tetrahydrofuran; N,N-dimethyl-formamide for 46h; Molecular sieve; Enzymatic reaction; regioselective reaction;55%
2635-84-9

p-nitrophenyl butyrate

82451-20-5

2'-p-hydroxybenzoyl mussaenosidic acid

1105707-88-7

6'-O-butanoyl-negundoside

Conditions
ConditionsYield
With Candida antarctica lipase B In tetrahydrofuran; N,N-dimethyl-formamide for 28h; Molecular sieve; Enzymatic reaction; regioselective reaction;50%
2635-84-9

p-nitrophenyl butyrate

(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)chroman-3-yl butyrate

Conditions
ConditionsYield
With carboxyesterase from Aspergillus niger; Mcllvaine buffer In acetone at 33℃; for 18h; pH=5; Acylation;35%
2635-84-9

p-nitrophenyl butyrate

94-41-7

benzalacetophenone

((1R,5R)-5-methylcyclopent-3-ene-1,3-diyl)dibenzene

Conditions
ConditionsYield
With (R)-5-neopentyl-2-phenyl 6,7-dihydro-5H-pyrrolo[2,1-c][1,2,4]triazol-2-ium tetrafluoroborate; 1,8-diazabicyclo[5.4.0]undec-7-ene In acetonitrile Molecular sieve;18%
616-47-7

1-methyl-1H-imidazole

2635-84-9

p-nitrophenyl butyrate

Butyric acid 4-nitro-phenyl ester; compound with 1-methyl-1H-imidazole

Conditions
ConditionsYield
With 2-(cyclohexylamino)ethanesulfonic acid In acetonitrile at 27℃; Equilibrium constant;
33966-50-6

SEC-BUTYLAMINE

2635-84-9

p-nitrophenyl butyrate

A

100-02-7

4-nitro-phenol

B

62124-64-5

N-sec-butyl-butyramide

Conditions
ConditionsYield
In acetonitrile at 25.4℃; Rate constant;
71-44-3

Spermine

2635-84-9

p-nitrophenyl butyrate

A

130306-92-2

N-{3-[4-(3-Amino-propylamino)-butylamino]-propyl}-butyramide

B

130306-94-4

N-{3-[4-(3-Butyrylamino-propylamino)-butylamino]-propyl}-butyramide

Conditions
ConditionsYield
In methanol Ambient temperature;
2635-84-9

p-nitrophenyl butyrate

A

14609-74-6

p-nitrophenolate

B

C40H66O31

Conditions
ConditionsYield
In methanol at 25℃; Mechanism; Rate constant; also with β-cyclodextrin;
2635-84-9

p-nitrophenyl butyrate

100-02-7

4-nitro-phenol

Conditions
ConditionsYield
With water; modified cyclodextrin In n-heptane Rate constant; also at other catalysts, other temperatures;
With lipase (extract from C.viscosum) In ethanol; water pH:8.0 (0.05 mol dm-3 tris buffer); var.: various lipase samples; influence of surfactant on KM and kcat;
With Na2CO3 buffer pH 10.4 In water; acetonitrile at 25℃; Rate constant; investigation of the hydrolytic cleavage of various p-nitrophenyl alkanoates in moderately alkaline aq. solution;
2635-84-9

p-nitrophenyl butyrate

14609-74-6

p-nitrophenolate

Conditions
ConditionsYield
With hydrogenchloride; buffer solution (0.01 M HEPES); sodium molybdate In acetonitrile at 27℃; Rate constant; Mechanism; hydrolysis;
With water; N-acetyl-L-histidine In acetonitrile at 31℃; Rate constant; var. catalysts - histidine derivatives, var. surfactants, pH 7.45;
With Psychrobacter sp. TA144 lipase, recombinant, containing MPILPV sequence at N-terminal, apparent molecular mass: ca. 48000 Da In acetonitrile at 35℃; pH=8; Kinetics; aq. buffer; Enzymatic reaction;
With {(25s,29s,33s,37s)-25,29,33,37-tetramethyl-2,4,8,10,14,16,20,22-octaoxanonacyclo[21.15.1.124,38.05,36.07,34.011,32.013,30.017,28.019,26]tetraconta-1(39),5(36),6,11(32),12,17(28),18,23,26,30,34,38(40)-dodecaene-6,12,18,39-tetrathio}tetrakis(acetyl-GGEQLLKQLEQLLKQHG-amide); water In aq. phosphate buffer at 20℃; pH=7; Kinetics; Reagent/catalyst;
With water In aq. phosphate buffer at 20℃; pH=7; Kinetics;
2635-84-9

p-nitrophenyl butyrate

A

14609-74-6

p-nitrophenolate

B

461-55-2

butyrate

Conditions
ConditionsYield
With (2-hydroxypropyl)-α-cyclodextrin In methanol; water Rate constant; phosphate buffer, pH 11.6;
2635-84-9

p-nitrophenyl butyrate

461-55-2

butyrate

Conditions
ConditionsYield
With Carbonate buffer In water at 25℃; Rate constant; investigation of the rate of hydrolysis in the absence of β-CD or γ-CD;
2635-84-9

p-nitrophenyl butyrate

A

100-02-7

4-nitro-phenol

B

107-92-6

butyric acid

Conditions
ConditionsYield
With alkaline solution In water; acetonitrile at 24.9 - 25.1℃; Rate constant; carbonate buffer pH 10.70;
With 1-methyl-1H-imidazole; 2-(cyclohexylamino)ethanesulfonic acid In acetonitrile at 27℃; Rate constant;
With Carbonate buffer; alpha cyclodextrin In water; dimethyl sulfoxide at 25℃; Rate constant; also with β-cyclodextrin;
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Specification

The Butanoic acid,4-nitrophenyl ester, with the CAS registry number of 2635-84-9, is also known as Butanoic acid p-nitrophenyl ester. The molecular formula of this chemical is C10H11NO4 and its molecular weight is 209.2. What's more, its IUPAC name is (4-Nitrophenyl) butanoate. In addition, it must be stored at 2-8 °C and placed in a dry, cool place.

Physical properties about Butanoic acid,4-nitrophenyl ester are: (1)ACD/LogP: 2.55; (2)# of Rule of 5 Violations: 0; (3)ACD/LogD (pH 5.5): 2.55; (4)ACD/LogD (pH 7.4): 2.55; (5)ACD/BCF (pH 5.5): 51.29; (6)ACD/BCF (pH 7.4): 51.29; (7)ACD/KOC (pH 5.5): 583; (8)ACD/KOC (pH 7.4): 583; (9)#H bond acceptors: 5; (10)#H bond donors: 0; (11)#Freely Rotating Bonds: 5; (12)Polar Surface Area: 72.12 Å2; (13)Index of Refraction: 1.533; (14)Molar Refractivity: 53.4 cm3; (15)Molar Volume: 171.9 cm3; (16)Polarizability: 21.17×10-24 cm3; (17)Surface Tension: 44.4 dyne/cm; (18)Density: 1.216 g/cm3; (19)Flash Point: 144.8 °C; (20)Enthalpy of Vaporization: 56.51 kJ/mol; (21)Boiling Point: 323.2 °C at 760 mmHg; (22)Vapour Pressure: 0.000266 mmHg at 25 °C.

Preparation of Butanoic acid,4-nitrophenyl ester: this chemical is prepared by reaction of Butyric acid anhydride with 4-Nitro-phenol at ambient temperature. This reaction needs reagents Dioxane and Pyridine. The reaction time is 2 hours. The yield is about 52 %.

The Butanoic acid,4-nitrophenyl ester can be obtained by Butyric acid anhydride and 4-Nitro-phenol.

Uses of Butanoic acid,4-nitrophenyl ester: it is used to produce other chemicals. For example, it is used to produce p-Aminophenylbutyrat. This reaction needs reagent Hydrogen, catalyst Platinum oxide and solvent Ethanol. The yield is about 93 %.

Butanoic acid,4-nitrophenyl ester can be used to produce p-Aminophenylbutyrat.

When you are using this chemical, please be cautious about it as the following:
As a chemical, it may cause sensitisation by skin contact. In addition, this chemical may cause inflammation to the skin or other mucous membranes. During using it, wear suitable protective clothing and gloves.

You can still convert the following datas into molecular structure:
(1) SMILES: O=C(Oc1ccc(cc1)[N+]([O-])=O)CCC
(2) InChI: InChI=1/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3
(3) InChIKey: DVDUMIQZEUTAGK-UHFFFAOYAM