1132-61-2

Basic information

• Product Name: 3-Morpholinopropanesulfonic acid
• Synonyms: MOPS;MOPS, CONCENTRATE SOLUTION;MORPHOLINOPROPANE SULFONIC ACID;TIMTEC-BB SBB009133;LABOTEST-BB LT00455082;LABOTEST-BB LT01595956;4-MORPHOLINEPROPANESULFONIC ACID;4-(MORPHOLINOPROPANE SULFONIC ACID)
• CAS NO: 1132-61-2
• Molecular Formula: C₇H₁₅NO₄S
• Molecular Weight: 209.26
• EINECS: 214-478-5
• Product Categories: Miscellaneous Biochemicals;Organic acids;Biochemistry;Good's Buffers;Buffer;Imidazoles
• Mol File: 1132-61-2.mol
• Article Data: 7

Chemical Properties

• Melting Point: 277-282 °C
• Flash Point: 116 °C
• Appearance: White/Powder/Solid
• Density: 1.3168 (rough estimate)
• Vapor Pressure: 0Pa at 25℃
• Refractive Index: 1.6370 (estimate)
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear
• PKA: 7.2(at 25℃)
• Water Solubility: 1000 g/L (20 ºC)
• Stability: Stable. Incompatible with strong bases, strong oxidizing agents.
• Merck: 14,6265
• BRN: 1106776
• CAS DataBase Reference: 3-Morpholinopropanesulfonic acid(CAS DataBase Reference)
• NIST Chemistry Reference: 3-Morpholinopropanesulfonic acid(1132-61-2)
• EPA Substance Registry System: 3-Morpholinopropanesulfonic acid(1132-61-2)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 1
• RTECS: QE9104530
• TSCA: Yes
• Hazardous Substances Data: 1132-61-2(Hazardous Substances Data)

Usage

Description

MOPS (3-morpholinopropanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.It is used as a synthetic buffering agent below pH 7.5.

application

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.

Reference

P. H. Quail, D. Marme, E. Sch?fer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191

Chemical Properties

White/clear crystalline powder

Uses

Different sources of media describe the Uses of 1132-61-2 differently. You can refer to the following data:
1. 3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.MOPS has been used as:a cell culture additive component in lentiviral particle production.as a buffering agent in microbial growth medium and nuclei extraction buffer.as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum.as a buffer in capillary-zone electrophoresis to test performance.for the dilution of proteins from algal samples.
2. MOPS acts as a multi-purpose buffering agent used in various biological research.
3. MOPS has been used as:a cell culture additive component in lentiviral particle productionas a buffering agent in microbial growth medium and nuclei extraction buffer

Definition

ChEBI: 3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.

General Description

3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.

Flammability and Explosibility

Notclassified

InChI:InChI=1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)

Synthetic route

morpholine (110-91-8) + 1,3-propanesultone (1120-71-4) → 3-(N-morpholino)propanesulfonic acid (1132-61-2)

Conditions	Yield
In acetone at 25℃; for 3.5h;	96%
In ethanol; water at 0℃; for 2h; Large scale;	81%

3-(N-morpholino)propanesulfonic acid (1132-61-2) → 3-(morpholin-4-yl)propane-1-sulfonyl chloride hydrochloride

Conditions	Yield
With thionyl chloride In N-methyl-acetamide
With thionyl chloride In N,N-dimethyl-formamide at 20℃; for 5h;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + D-glucose (50-99-7) + caesin, acid hydrolyzed + extract of yeast + soy peptone, type SL + L-<3-(13)C>serine (89232-77-9) → C46(13)C15H60N14O18S5

Conditions	Yield
With Amycolatopsis fastidiosa MA7344 In water at 32℃; for 168h; Microbiological reaction;

3-(N-morpholino)propanesulfonic acid (1132-61-2) → C2H5O4S(1-)*C2H6NO3S(1-)*C7H14NO4S(1-)*3C7H15N2(1+)

Conditions	Yield
Multi-step reaction with 2 steps 1: water 2: water View Scheme

3-(N-morpholino)propanesulfonic acid (1132-61-2) + N-methyltriethylenediamine hydroxide → [C1ted][MOPS] (1416049-31-4)

Conditions	Yield
In water

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetra-n-butylphosphonium hydroxide (14518-69-5) → [TBP][MOPS]

Conditions	Yield
In water at 20℃;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetramethyl ammoniumhydroxide (75-59-2) → C7H14NO4S(1-)*C4H12N(1+)

Conditions	Yield
In water at 20℃; for 12h;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetraethylammonium hydroxide (77-98-5) → C7H14NO4S(1-)*C8H20N(1+)

Conditions	Yield
In water at 20℃; for 12h;

3-(N-morpholino)propanesulfonic acid (1132-61-2) + tetra(n-butyl)ammonium hydroxide (2052-49-5) → 3-Morpholin-4-yl-propane-1-sulfonatetetrabutyl-ammonium; (113599-00-1)

Conditions	Yield
In water at 20℃; for 12h;

Upstream product

• 1132610-98-0 1H-Pyrrolo[2,3-b]pyridine-2-carboxylic acid, 5-chloro-3-iodo-, ethyl ester 
• 1132610-92-4 Methyl 5-Methoxy-6-azaindole-2-carboxylate 
• 1132610-85-5 3-BROMO-5-METHYL-4-AZAINDOLE-2-CARBOXYLIC ACID ETHYL ESTER 
• 1132610-84-4 ethyl 5-Methyl-1H-pyrrolo[3,2-b]pyridine-2-carboxylate 
• 1132610-60-6 Phosphonic acid, P-[(5-fluoro-6-Methyl-2-pyridinyl)(phenylaMino)Methyl]-, diphenyl ester 
• 1132610-54-8 Ethanone, 2-broMo-1-(5-fluoro-6-Methyl-2-pyridinyl)-2-(6-quinoxalinyl)- 
• 1132610-47-9 Ethanone, 1-(5-fluoro-6-Methyl-2-pyridinyl)-2-[1,2,4]triazolo[1,5-a]pyridin-6-yl- 
• 1132610-46-8 1H-IMidazol-2-aMine, 5-(5-fluoro-6-Methyl-2-pyridinyl)-4-(6-quinoxalinyl)- 
• 1132610-45-7 AcetaMide, N-[5-(5-fluoro-6-Methyl-2-pyridinyl)-4-(6-quinoxalinyl)-1H-iMidazol-2-yl]- 
• 1132610-44-6 Ethanone, 1-(5-fluoro-6-Methyl-2-pyridinyl)-2-(6-quinoxalinyl)- 
• 113261-00-0 (3-AMINO-PHENYL)-CARBAMIC ACID BENZYL ESTER 
• 113260-74-5 N,N?BIS(4-ISOPROPYLPHENYL) UREA 
• 1132-60-1 piperidine-1-propanesulphonic acid 
• 113258-90-5 4-hydroxy-2-methyl-4-phenyl-1,2,3,4-tetrahydroisoquinoline 

Downstream Products

• 1132638-93-7 1H-1,2,3-benzotriazole-5-carboxylic acid, 7-Methyl-, Methyl ester 
• 1132638-95-9 1,3-Dimethyl-2-oxo-1,2-dihydroquinoxaline-6-carboxylic acid 
• 1132639-46-3 oxane-2,3,4,5-tetrol 
• 113264-13-4 4-(chloromethyl)-2-(4-fluorophenyl)-1,3-thiazole(SALTDATA: FREE) 
• 113264-15-6 2-butyl-4-(chloromethyl)-1,3-thiazole 
• 113264-43-0 3-BroMo-2-oxo-pentanoic acid ethyl ester 
• 1132649-21-8 3-Butanoyl-1-tosylpyrrole 
• 1132650-51-1 Ethyl 6-(Trifluoromethyl)-1-benzothiophene-2-carboxylate 
• 1132650-67-9 Lisinopril Cyclohexyl Analog 
• 1132653-79-2 Robotnikinin 
• 113265-41-1 6-nitro-2-(4-methylphenyl)imidazo[1,2-a]pyridine 
• 113265-44-4 2-METHYL-6-(TRIFLUOROMETHYL)PYRIDINE-3-METHANOL 
• 1132654-54-6 Lurasidone-d8 Hydrochloride 
• 1132656-73-5 (R)-Ostarine 

Recommend Products

• 197891-91-1  3-(morpholin-4-yl)propane-1-sulfonyl chloride hydrochloride 
• 110-91-8  morpholine 
• 1120-71-4  1,3-propanesultone