1241390-29-3

Usage

Uses

Used in Biological Assays:
DHFBI is used as a fluorescent marker in various biological assays. Its chemical structure allows it to mimic the properties of green fluorescent protein (GFP), making it a valuable tool for tracking and visualizing cellular processes, protein localization, and gene expression.
Used in Drug Development:
In the pharmaceutical industry, DHFBI is utilized as a key component in the development of new drugs. Its fluorescence properties enable researchers to monitor the effects of drug candidates on cellular processes and to study the interactions between drugs and their target molecules. This helps in the identification of potential therapeutic agents and the optimization of drug efficacy and safety.
Used in Research and Development:
DHFBI is also employed in research and development across various scientific disciplines. Its ability to mimic GFP makes it a versatile tool for studying cellular and molecular processes, as well as for developing new diagnostic and imaging techniques. Researchers can use DHFBI to label and track specific proteins, monitor cellular signaling pathways, and investigate the mechanisms of disease progression.

Biochem/physiol Actions

3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) is non fluorescent when unbound.

Upstream product

• 1332478-75-7 (Z)-2,6-difluoro-4-((2-methyl-5-oxooxazol-4(5H)-ylidene)methyl)phenyl acetate 
• 74-89-5 methylamine 
• 123-75-1 pyrrolidine 
• 67-56-1 methanol 
• 118276-06-5 3,5-difluoro-4-hydroxybenzaldehyde 

Relevant academic research and scientific papers

Understanding the photophysics of the Spinach-DFHBI RNA aptamer-fluorogen complex to improve live-cell RNA imaging

The use of aptamer-fluorogen complexes is an emerging strategy for RNA imaging. Despite its promise for cellular imaging and sensing, the low fluorescence intensity of the Spinach-DFHBI RNA aptamer-fluorogen complex hampers its utility in quantitative liv

Bifacial peptide nucleic acid as an allosteric switch for aptamer and ribozyme function

We demonstrate herein that bifacial peptide nucleic acid (bPNA) hybrid triplexes functionally substitute for duplex DNA or RNA. Structure-function loss in three non-coding nucleic acids was inflicted by replacement of a duplex stem with unstructured oligo-T/U strands, which are bPNA binding sites. Functional rescue was observed on refolding of the oligo-T/U strands into bPNA triplex hybrid stems. Bifacial PNA binding was thus used to allosterically switch-on protein and small-molecule binding in DNA and RNA aptamers, as well as catalytic bond scission in a ribozyme. Duplex stems that support the catalytic site of a minimal type I hammerhead ribozyme were replaced with oligo-U loops, severely crippling or ablating the native RNA splicing function. Refolding of the U-loops into bPNA triplex stems completely restored splicing function in the hybrid system. These studies indicate that bPNA may have general utility as an allosteric trigger for a wide range of functions in non-coding nucleic acids.

Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores

A genetically encoded fluorescent tag for live cell microscopy is presented. This tag is composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative of green fluorescent protein (GFP)-like chromophore with red fluorescence. The reversible binding of the novel fluorogen and FAST is accompanied by three orders of magnitude increase in red fluorescence (580–650 nm). The proposed dye instantly stains target cellular proteins fused with FAST, washes out in a minute timescale, and exhibits higher photostability of the fluorescence signal in confocal and widefield microscopy, in contrast with previously published fluorogen:FAST complexes.

Plug-and-play fluorophores extend the spectral properties of spinach

Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (DFHBI), allowing the dynamic localizations of Spinach2-tagged RNAs to be imaged in live cells. The spectral properties of Spinach2 are limited by DFHBI, which produces fluorescence that is bluish-green and is not optimized for filters commonly used in fluorescence microscopes. Here we characterize the structural features that are required for fluorophore binding to Spinach2 and describe novel fluorophores that bind and are switched to a fluorescent state by Spinach2. These diverse Spinach2-fluorophore complexes exhibit fluorescence that is more compatible with existing microscopy filter sets and allows Spinach2-tagged constructs to be imaged with either GFP or YFP filter cubes. Thus, these plug-and-play fluorophores allow the spectral properties of Spinach2 to be altered on the basis of the specific spectral needs of the experiment.