1246401-08-0Relevant academic research and scientific papers
Design of red-shifted and environment-sensitive fluorogens based on GFP chromophore core
Baranov, Mikhail S.,Mishin, Alexander S.,Perfilov, Maxim M.,Smirnov, Alexander Yu.,Zagudaylova, Marina B.,Zaitseva, Elvira R.,Zaitseva, Snizhana O.
, (2020)
Fluorescent staining is an indispensable method to study living systems. One of the recent fundamental advances in this field is the development of fluorogenic dyes - compounds that have no pronounced fluorescence in the free state but acquire it upon binding with the target object, allowing for simple no-wash labeling. Among these dyes, it is worth to note compounds whose fluorescence is particularly sensitive to the properties of the microenvironment (e.g., solvent), which can be used, for example, for staining cell organelles. In this paper, we present a novel technique for the creation of such environment-sensitive and red-shifted fluorogenic dyes with large Stokes shifts. Novel dyes are based on the GFP chromophore core and exhibit high solvent-dependent variation of emission maxima position and fluorescence quantum yield. These compounds are cell-permeable and rapidly stain the endoplasmic reticulum in living cells.
Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores
Povarova, Natalia V.,Zaitseva, Snizhana O.,Baleeva, Nadezhda S.,Smirnov, Alexander Yu.,Myasnyanko, Ivan N.,Zagudaylova, Marina B.,Bozhanova, Nina G.,Gorbachev, Dmitriy A.,Malyshevskaya, Kseniya K.,Gavrikov, Alexey S.,Mishin, Alexander S.,Baranov, Mikhail S.
supporting information, p. 9592 - 9596 (2019/07/09)
A genetically encoded fluorescent tag for live cell microscopy is presented. This tag is composed of previously published fluorogen-activating protein FAST and a novel fluorogenic derivative of green fluorescent protein (GFP)-like chromophore with red fluorescence. The reversible binding of the novel fluorogen and FAST is accompanied by three orders of magnitude increase in red fluorescence (580–650 nm). The proposed dye instantly stains target cellular proteins fused with FAST, washes out in a minute timescale, and exhibits higher photostability of the fluorescence signal in confocal and widefield microscopy, in contrast with previously published fluorogen:FAST complexes.
Excited-state intramolecular proton transfer molecules bearing o -hydroxy analogues of green fluorescent protein chromophore
Chuang, Wei-Ti,Hsieh, Cheng-Chih,Lai, Chin-Hung,Lai, Cheng-Hsuan,Shih, Chun-Wei,Chen, Kew-Yu,Hung, Wen-Yi,Hsu, Yu-Hsiang,Chou, Pi-Tai
experimental part, p. 8189 - 8202 (2011/12/04)
o-Hydroxy analogues, 1a-g, of the green fluorescent protein chromophore have been synthesized. Their structures and electronic properties were investigated by X-ray single-crystal analyses, electrochemistry, and luminescence properties. In solid and nonpo
