16182-04-0Relevant articles and documents
Lipid membrane adhesion and fusion driven by designed, minimally multivalent hydrogen-bonding lipids
Ma, Mingming,Gong, Yun,Bong, Dennis
, p. 16919 - 16926 (2009)
Cyanuric acid (CA) and melamine (M) functionalized lipids can form membranes that exhibit robust hydrogen-bond driven surface recognition in water, facilitated by multivalent surface clustering of recognition groups and variable hydration at the lipid-water interface. Here we describe a minimal lipid recognition cluster: three CA or M recognition groups are forced into proximity by covalent attachment to a single lipid headgroup. This trivalent lipid system guides recognition at the lipid-water interface using cyanurate-melamine hydrogen bonding when incorporated at 0.1-5 mol percent in fluid phospholipid membranes, inducing both vesicle-vesicle binding and membrane fusion. Fusion was accelerated when the antimicrobial peptide magainin was used to anchor trivalent recognition, or when added exogenously to a preassembled lipid vesicle complex, underscoring the importance of coupling recognition with membrane disruption in membrane fusion. Membrane apposition and fusion were studied in vesicle suspensions using light scattering, FRET assays for lipid mixing, surface plasmon resonance, and cryo-electron microscopy. Recognition was found to be highly spatially selective as judged by vesicular adhesion to surface patterned supported lipid bilayers (SLBs). Fusion to SLBs was also readily observed by fluorescence microscopy. Together, these studies indicate effective and functional recognition of trivalent phospholipids, despite low mole percentage concentration, solvent competition for hydrogen bond donor/acceptor sites, and simplicity of structure. This novel designed molecular recognition motif may be useful for directing aqueous-phase assembly and biomolecular interactions.
Visualized detection of melamine in milk by supramolecular hydrogelations
Zhang, Jianwu,Ou, Caiwen,Shi, Yang,Wang, Ling,Chen, Minsheng,Yang, Zhimou
, p. 12873 - 12876 (2014)
We reported a visualized detection system for melamine based on supramolecular dydrogelations.
Fusion reactions of N-heterocyclic moieties to thiopyrano[4′,3′:4,5]thieno[2,3-d]pyrimidines
Ahmed, Essam K.,Froehlich, Johannes,Sauter, Fritz
, p. 147 - 154 (1996)
Derivatives of the novel heterocyclic parent systems imidazolo[1,2-a]thiopyrano[4′,3′:4,5]thieno[2,3-d]-pyrimidine (B) and thiopyrano[4′,3′:4,5]thieno[3,2-e][1,2,4]triazolo[1,5-c]pyrimidine (C) have been synthesized by fusing pyrimidine moieties to 2-amino-4,7-dihydro-5H-thieno[2,3-c]thiopyran-3-car-boxylic acid ethyl ester (1) and -3-carbonitrile (10), followed by cyclization reactions of the title intermediates A thus obtained.
Structural, Hirshfeld surface and in?vitro cytotoxicity evaluation of five new N-aryl-N’-alkoxycarbonyl thiocarbamide derivatives
Pandey, Sunil K.,Pratap, Seema,Rai, Sunil K.,Marverti, Gaetano
, p. 812 - 820 (2020)
Five new compounds, N-(2, 4-dichlorophenyl)-N’-(methoxycarbonyl) thiocarbamide (1), N-(2, 4-dichlorophenyl)-N’-(ethoxycarbonyl) thiocarbamide (2), N-(2, 4-dichlorophenyl)-N’-(2, 2, 2-trichloroethoxycarbonyl) thiocarbamide (3), N-(2,4-dichlrophenyl)-N’-(pentoxycarbonyl) thiocarbamide (4) and N-(4-nitrophenyl)-N’-(pentoxycarbonyl) thiocarbamide (5), have been synthesized by the reaction of various alkoxy chloroformates with 2, 4-dichloroaniline/4-nitroaniline.The molecular structures of the compounds were elucidated by using spectroscopic methods (FT-IR, 1H and 13C NMR) and single-crystal X-ray structure analysis of compounds 2 and 5. Antiperiplanar orientation of C = O and C = S group across C–N bonds of thiocarbamide core may be due to the presence of intramolecular (N–H···O–C) hydrogen bond in the crystal structure of both the compounds. The presence of intermolecular interactions (C–H···S, C–H···O and N–H···S) in the molecular structure of the compounds has been studied in detail using Hirshfeld surfaces and their associated two-dimensional fingerprint plots. In vitro cytotoxicity screening of the synthesized compounds evaluated on a panel of seven human cancer cell lines (cervical carcinoma (2008, C13*), colorectal (HT29 and HCT116) and ovarian carcinoma (A2780, A2780/CP and IGROV-1)) demonstrated significant inhibitory properties.
Preparation process of alkoxycarbonyl thiourea
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Paragraph 0025-0026; 0028-0029, (2021/01/24)
The invention relates to a preparation process of alkoxycarbonyl thiourea. The process comprises the following steps: 1, preparing alkoxycarbonyl isothiocyanate by using chloroformate and thiocyanateas raw materials; and 2, adding an aqueous solution of an amine compound to the bottom of a reaction kettle, slowly dropwise adding the alkoxycarbonyl isothiocyanate prepared in the step 1, controlling the dropwise adding temperature at 0-50 DEG C, continuously heating to 60-90 DEG C after the dropwise adding is completed, and standing for layering to obtain an organic phase product. According tothe preparation process of the alkoxycarbonyl thiourea, the feeding mode is changed, and production operation control is better facilitated; the heat release rate of the alkoxycarbonyl thiourea synthesis reaction is favorably reduced; safe production is facilitated; and no flammable and explosive organic solvent with high cost and large pollution is used. The process is simple to operate, and is more beneficial to industrial production control operation, so that safe production of the alkoxycarbonyl thiourea is realized.
Synthesis, crystal structure and herbicidal activity of a series of [1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide compounds
Ma, Yi-Chao,Yu, Ying-Hui,Hou, Guang-Feng,Huang, Ji-Han,Gao, Jin-Sheng
, p. 829 - 843 (2016/07/06)
With the aim of obtaining efficient, safe and environmentally friendly green herbicide, a series of [1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide compounds (8a-8f) were synthesized by reacting 2-amino-5,7-(bis-substituted)- 1,2,4-triazolo[1,5-a]pyrimidine (4a and 4b) with 2-substituted-6-trifluoromethyl-benzenesulfonyl chloride (7a-7c). And their structures were characterized by H-NMR, C-NMR, HRMS, FTIR, single-crystal X-ray diffraction, elemental analysis. Moreover, their herbicidal activities against six species of weeds were evaluated. Three target compounds such as 8a, 8c and 8e, exhibited significant postemergence herbicidal activity against some common dicotyledons and monocotyledons under different concentrations. The structure and activity relationship is discussed based on the herbicidal performances of the compounds with different substituents. The investigation results indicated that the above structures could serve as lead compounds for the development of new herbicides.