201467-81-4Relevant articles and documents
Immobilization of liposomes and vesicles on patterned surfaces by a peptide coiled-coil binding motif
Voskuhl, Jens,Wendeln, Christian,Versluis, Frank,Fritz, Eva-Corinna,Roling, Oliver,Zope, Harshal,Schulz, Christian,Rinnen, Stefan,Arlinghaus, Heinrich F.,Ravoo, Bart Jan,Kros, Alexander
, p. 12616 - 12620 (2012)
Patchy surfaces: An azide-terminated self-assembled monolayer was patterned with the peptide sequence (EIAALEK)3 by using microcontact printing. This sequence forms stable coiled-coil heterodimers with the complementary peptide (KIAALKE)3
TARGETED BIFUNCTIONAL DEGRADERS
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, (2021/04/17)
The present invention provides, in one aspect, bifunctional compounds that can be used to promote or enhance degradation of certain circulating proteins. In another aspect, the present invention provides bifunctional compounds that can be used to promote or enhance degradation of certain autoantibodies. In certain embodiments, treatment or management of a disease and/or disorder requires degradation, removal, or reduction in concentration of the circulating protein or the autoantibody in the subject. Thus, in certain embodiments, administration of a compound of the invention to the subject removes or reduces the circulation concentration of the circulating protein or the autoantibody, thus treating, ameliorating, or preventing the disease and/or disorder. In certain embodiments, the circulating protein is TNF.
Optical Manipulation of Subcellular Protein Translocation Using a Photoactivatable Covalent Labeling System
Kowada, Toshiyuki,Arai, Keisuke,Yoshimura, Akimasa,Matsui, Toshitaka,Kikuchi, Kazuya,Mizukami, Shin
, p. 11378 - 11383 (2021/04/09)
The photoactivatable chemically induced dimerization (photo-CID) technique for tag-fused proteins is one of the most promising methods for regulating subcellular protein translocations and protein–protein interactions. However, light-induced covalent protein dimerization in living cells has yet to be established, despite its various advantages. Herein, we developed a photoactivatable covalent protein-labeling technology by applying a caged ligand to the BL-tag system, a covalent protein labeling system that uses mutant β-lactamase. We further developed CBHD, a caged protein dimerizer, using caged BL-tag and HaloTag ligands, and achieved light-induced protein translocation from the cytoplasm to subcellular regions. In addition, this covalent photo-CID system enabled quick protein translocation to a laser-illuminated microregion. These results indicate that the covalent photo-CID system will expand the scope of CID applications in the optical manipulation of cellular functions.
