20300-59-8

Usage

Uses

Used in Molecular Biology:
7-METHOXYCOUMARIN-3-CARBOXYLIC ACID is used as a labelling agent for oligonucleotides and ribonucleotides. This application is crucial in molecular biology as it allows for the detection and analysis of specific nucleic acid sequences, which are essential for understanding gene expression, regulation, and function.
Used in Fluorescence Applications:
7-METHOXYCOUMARIN-3-CARBOXYLIC ACID is used as a contrast agent in multicolor fluorescence applications. It provides a distinguishable contrast when used with longer-wavelength probes, making it suitable for various techniques such as nucleic acid and protein microarrays, in situ hybridization, and immunofluorescence. This application is valuable in the field of biochemistry and molecular biology, as it enables researchers to study the interactions between different biomolecules and their roles in cellular processes.

InChI:InChI=1/C11H8O5/c1-15-7-3-2-6-4-8(10(12)13)11(14)16-9(6)5-7/h2-5H,1H3,(H,12,13)

Upstream product

• 673-22-3 2-Hydroxy-4-methoxybenzaldehyde 
• 2033-24-1 cycl-isopropylidene malonate 
• 141-82-2 malonic acid 
• 613-45-6 2,4-Dimethoxybenzaldehyde 
• 13229-92-0 7-methoxy-2-oxo-2H-chromene-3-carbonitrile 
• 6093-71-6 7-hydroxy-2-oxo-2H-chromene-3-carboxylic acid ethyl ester 
• 95-01-2 2,4-Dihydroxybenzaldehyde 
• 105-53-3 diethyl malonate 
• 150-19-6 O-methylresorcine 
• 86788-49-0 7-hydroxy-2-oxo-2H-chromene-3-carboxylic acid methyl ester 
• 133031-79-5 dipotassium 2,5-dimethoxybenzylidenemalonate 
• 7324-87-0 diethyl 2-(2,5-dimethoxybenzylidene)malonate 
• 682805-45-4 (2,5-dimethoxy-benzylidene)-malonic acid 
• 794517-54-7 2-imino-7-methoxy-2H-1-benzopyran-3-carbonitrile 

Downstream Products

• 51867-62-0 7-methoxy-2-oxo-2H-chromene-3-carbonyl chloride 
• 871927-37-6 3-(4-Ethoxy-2-oxocyclohex-3-enyl)propyl-7-methoxy-2-oxo-2H-chromen-3-ylcarbamate 
• 2555-22-8 3-phenyl-7-methoxycoumarin 
• 1366125-58-7 C₁₈H₁₆O₄S 
• 1262794-02-4 7-methoxy-3-(3,5-dinitrobenzyl)aminocarbonylcoumarin 
• 2555-31-9 7-methoxy-4-phenyl-2H-chromen-2-one 
• 51868-52-1 7-methoxy-3-(3-phenyl-1,2,4-oxadiazol-5-yl)-coumarin 
• 51868-54-3 7-methoxy-3-[3-(4-methoxyphenyl)-1,2,4-oxadiazol-5-yl]coumarin 
• 1350629-69-4 7-methoxycoumarin-3-carboxylic acid 2,3,4,5,6-pentafluorophenyl ester 

Relevant academic research and scientific papers

Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound

Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface a

A Simple One-Pot Synthesis of Fully Substituted 1H-Pyridone[1,2-a]-Fused-1,3-Diazaheterocycles

An efficient procedure for the synthesis of 7-(aryl)-8-nitro-2,3,6,7-tetrahydroimidazo[1,2-a]pyridinones, 8-(aryl)-9-nitro-3,4,7,8-tetrahydropyridone[1,2-a]pyrimidines and 9-(aryl)-10-nitro-2,3,4,5,8,9-hexahydropyridone[1,2-a]diazepine via one-pot three component reaction of diamine, nitroketene dithioacetal (1,1-bis(methylsulfanyl)-2-nitroethene), and coumarine-3- carboxylic acid derivatives in EtOH under reflux conditions is reported. The advantages of this procedure are simplicity, easy purification, good yields, and catalyst-free conditions. All products were confirmed by 1H- and 13C-NMR, IR, MS, and X-ray crystal structure analyses.

ZrOCl2 · 8H2O: An efficient catalyst for rapid one-pot synthesis of 3-carboxycoumarins under ultrasound irradiation in water

In this report, a rapid, efficient and environmental friendly synthesis of 3-carboxycoumarins under ultrasound irradiation in water media is described. The Knoevenagel condensation of Meldrum's acid with substituted benzaldehydes in the presence of zirconium oxide chloride (ZrOCl2·8H2O) and ultrasonic irradiation in water gave the corresponding 3-carboxycoumarins in good to excellent yields.

MESOMORPHIC HETEROCYCLIC HOMOLOGOUS SERIES: I. 7-(4 prime -n-ALKOXYBENZOYLOXY)-3-ACETYLCOUMARINS II. 4 prime -FORMYLPHENYL 7-n-ALKOXYCOUMARIN-3-CARBOXYLATES.

Two homologous series of coumarin derivatives have been synthesized and mesomorphic behavior of their members has been studied. In case of Series I, mesomorphism does not begin to appear until the butyl homolog, which is monotropic nematic. The remaining

A practical and environmentally friendly preparation of 3-carboxycoumarins

The condensation of salicylaldehydes with Meldrum's acid in water at 75°C to produce 3-carboxycoumarin derivatives is described.

Silica sulfuric acid: A versatile and reusable catalyst for synthesis of coumarin-3-carboxylic acids in a solventless system

Synthesis of substituted coumarin-3-carboxylic acid via Knoevenagel condensation of meldrum's acid with ortho-hydroxyaryl aldehydes in solventless system is described.

Solid phase synthesis of substituted coumarin-3-carboxylic acids via the Knoevenagel condensation

A solid phase synthesis of substituted coumarin-3-carboxylates using the Knoevenagel condensation reaction between ethyl malonate bound to the Wang resin and ortho-hydroxyarylaldehydes is described. The reaction has been shown to proceed cleanly to give the desired products.

Synthesis of new fluorophores derived from monoazacrown ethers and coumarin nucleus

New ionophores derived from azacrown ethers attached to coumarins have been synthesized and characterized. The alkaline-earth complexes of these new ligands were studied from their UV-vis, NMR, and fluorescence data. Some systems displayed bathochromic shifts and fluorescence decreases upon complexation with Ca2+ that may make them useful signaling devices of this metal.

A convenient synthesis of coumarin-3-carboxylic acids via Knoevenagel condensation of Meldrum's acid with ortho-hydroxyaryl aldehydes or ketones

Coumarin-3-carboxylic acids were obtained in high yields with excellent purity from ortho-hydroxybenzaldehydes and Meldrum's acid after a 2 h reflux in ethanol. The less reactive ketones were first reacted with alcoholic ammonia to form ketimines, which were then condensed with Meldrum's acid to generate 4-alkylcoumarin-3-carboxylic acids in moderate yields.

Method for synthesizing coumarin-3-carboxylic acid compounds by one-pot two-step method

The invention relates to a method for synthesizing coumarin-3-carboxylic acid compounds by a one-pot two-step method. The structural formula of the coumarin-3-carboxylic acid compounds is shown in the description, wherein R1 is H , Cl , Br, NO2 , CH3 and HO . According to the synthesis process of the coumarin-3-carboxylic acid compounds, malonic acid, acetone and substituted salicylaldehyde serve as raw materials, iodine serves as a catalyst, acetic anhydride serves as a solvent, a series of coumarin-3-carboxylic acid compounds are synthesized through a one-pot cascade reaction, and the efficiency and the yield are greatly improved compared with a fractional step method.