4264-83-9 Usage
Description
Disodium 4-nitrophenylphosphate (PNPP) is a colorimetric alkaline phosphatase soluble substrate that is widely used in various biochemical and diagnostic applications. It is a white crystalline compound with excellent solubility properties and is known for its ability to produce a yellow soluble end product when used with alkaline phosphatase. Disodium 4-nitrophenylphosphate has a maximum molar absorptivity (ε max) of ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3, making it an ideal choice for various assays and tests.
Uses
Used in Enzyme Linked Immunosorbant Assay (ELISA) Procedures:
Disodium 4-nitrophenylphosphate is used as a substrate for alkaline phosphatase in ELISA procedures. It serves as a reliable and sensitive indicator for the detection and quantification of specific antigens or antibodies in a sample. The yellow soluble end product produced can be read at 405 nm to 410 nm, allowing for both kinetic and endpoint determinations.
Used in Alkaline Phosphatase-based Assays:
Disodium 4-nitrophenylphosphate is used as an excellent substrate for alkaline phosphatase-based assays, providing a simple and efficient method for detecting and measuring the activity of the enzyme. The yellow end product generated can be easily measured using a spectrophotometer, making it a popular choice for researchers and diagnostic laboratories.
Used in Diagnostic Applications:
In the field of diagnostics, Disodium 4-nitrophenylphosphate is used as a colorimetric substrate to detect and measure the presence of alkaline phosphatase, an enzyme that is often elevated in certain disease states. Disodium 4-nitrophenylphosphate's ability to produce a visible color change upon enzyme activity makes it a valuable tool for diagnosing and monitoring various conditions, such as liver and bone disorders.
Used in Research and Development:
Disodium 4-nitrophenylphosphate is also used in research and development for the study of alkaline phosphatase and its role in various biological processes. Disodium 4-nitrophenylphosphate's properties make it an ideal tool for investigating enzyme kinetics, enzyme inhibition, and the development of new drugs and therapies targeting alkaline phosphatase.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, Disodium 4-nitrophenylphosphate is used as a reagent for the development and quality control of drugs that target alkaline phosphatase. Its ability to produce a measurable end product makes it a valuable tool for assessing the efficacy and safety of new drug candidates.
Used in Environmental Testing:
Disodium 4-nitrophenylphosphate is also used in environmental testing to detect and measure the presence of alkaline phosphatase in soil, water, and other samples. This information can be used to assess the health of ecosystems and monitor the effectiveness of environmental remediation efforts.
Biochem/physiol Actions
Primary TargetSubstrate for phosphatase
Purification Methods
Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.]
Check Digit Verification of cas no
The CAS Registry Mumber 4264-83-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,2,6 and 4 respectively; the second part has 2 digits, 8 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 4264-83:
(6*4)+(5*2)+(4*6)+(3*4)+(2*8)+(1*3)=89
89 % 10 = 9
So 4264-83-9 is a valid CAS Registry Number.
InChI:InChI=1/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
4264-83-9Relevant articles and documents
Enhanced hydrolytic activity of Cu(II) and Zn(II) complexes in highly cross-linked polymers
Schiller, Alexander,Scopelliti, Rosario,Severin, Kay
, p. 3858 - 3867 (2006)
The chelate ligand tris[(1-vinylimidazol-2-yl)methyl]amine (5) was synthesized in five steps from commercially available starting materials. Upon reaction with ZnCl2 or CuCl2 in the presence of NH 4PF6, the complexes [Zn(5)Cl]PF6 (6) and [Cu(5)Cl]PF6 (7) were obtained. The structure of both complexes was determined by single-crystal X-ray crystallography. Immobilization of 6 and 7 was achieved by co-polymerization with ethylene glycol dimethacrylate. The supported complexes P6-Zn and P7-Cu were found to be efficient catalysts for the hydrolysis of bis(p-nitrophenyl)phosphate (BNPP) at 50 °C. At pH 9.5, the heterogeneous catalyst P7-Cu was 56 times more active than the homogeneous catalyst 7. Partitioning effects, which increase the local concentration of BNPP in the polymer, are shown to contribute to the enhanced activity of the immobilized catalyst. The Royal Society of Chemistry 2006.
Solution speciation of the dinuclear ZrIV-substituted keggin polyoxometalate [{α-PW11O39Zr(μ-OH)(H2O)}2]8- and Its reactivity towards DNA-model phosphodiester hydrolysis
Luong, Thi Kim Nga,Absillis, Gregory,Shestakova, Pavletta,Parac-Vogt, Tatjana N.
supporting information, p. 5276 - 5284 (2015/04/22)
The solution speciation of the ZrIV-substituted Keggin polyoxometalate (Et2NH2)8[{α-PW11O39Zr(μ-OH)(H2O)}2]·7H2O (ZrK 2:2) was fully determined under differ