49621-03-6Relevant articles and documents
S-allylcysteine scavenges singlet oxygen and hypochlorous acid and protects LLC-PK1 cells of potassium dichromate-induced toxicity
Medina-Campos, Omar Noel,Barrera, Diana,Segoviano-Murillo, Sabina,Rocha, Diana,Maldonado, Perla D.,Mendoza-Patino, Nicandro,Pedraza-Chaverri, Jose
, p. 2030 - 2039 (2007)
It has been found that S-allylcysteine (SAC), a garlic-derived compound, has in vivo and in vitro antioxidant properties. In addition, it is known that SAC is able to scavenge different reactive oxygen or nitrogen species including superoxide anion (O2{radical dot} -), hydrogen peroxide (H2O2), hydroxyl radical (OH{radical dot}), and peroxynitrite anion (ONOO-) although the IC5O values for each reactive species has not been calculated and the potential ability of SAC to scavenge singlet oxygen (1O2) and hypochlorous acid (HOCl) has not been explored. The purposes of this work was (a) to explore the potential ability of SAC to scavenge 1O2 and HOCl, (b) to further characterize the O2{radical dot} -, H2O2, OH{radical dot}, and ONOO- scavenging ability of SAC by measuring the IC50 values using in vitro assays, and (c) to explore the potential ability of SAC to ameliorate the potassium dichromate (K2Cr2O7)-induced cytotoxicity in LLC-PK1 cells in which oxidative stress is involved. The scavenging activity was compared against the following reference compounds: N-acetylcysteine for O2{radical dot} -, sodium pyruvate for H2O2, dimethylthiourea for OH{radical dot}, lipoic acid and glutathione for 1O2, lipoic acid for HOCl, and penicillamine for ONOO-. It was found that SAC was able to scavenge concentration-dependently all the species assayed with the following IC5O (mean ± SEM, mM): O2{radical dot} - (14.49 ± 1.67), H2O2 (68 ± 1.92), OH{radical dot} (0.68 ± 0.06), 1O2 (1.93 ± 0.27), HOCl (2.86 ± 0.15), and ONOO- (0.80 ± 0.05). When the ability of SAC to scavenge these species was compared to those of the reference compounds it was found that the efficacy of SAC (a) to scavenge O2{radical dot} -, H2O2, OH{radical dot}, and ONOO- was lower, (b) to scavenge HOCl was similar, and (c) to scavenge 1O2 was higher. In addition, it was found that SAC was able to prevent K2Cr2O7-induced toxicity in LLC-PK1 cells in culture. It was showed for the first time that SAC is able to scavenge 1O2 and HOCl and to ameliorate the K2Cr2O7-induced toxicity.
A new dipeptide isolated from the bulb of garlic
Zhou, Mei-Yun,Wu, Zhao-Jun,Li, Wen-Qing,Hu, Xing-Peng,Yan, Rian,Ou, Shi-Yi,Zhou, Hua
, p. 323 - 326 (2014)
(R)-3-(allylthio)-2-((R)-3-(allylthio)-2-aminopropanamido)propanoic acid was isolated from the bulb of garlic, together with four known amino acids. Its structure was elucidated on the basis of 2D NMR and MS techniques. To the best of our knowledge, (R)-3-(allylthio)-2-((R)-3-(allylthio)-2-aminopropanamido) propanoic acid, which showed antibacterial activity against the Staphylococcus aureus antibiotic resistant strain, was the first example of dipeptide from garlic.
Expanding the Structural Diversity of Protein Building Blocks with Noncanonical Amino Acids Biosynthesized from Aromatic Thiols
Wang, Yong,Chen, Xiaoxu,Cai, Wenkang,Tan, Linzhi,Yu, Yutong,Han, Boyang,Li, Yuxuan,Xie, Yuanzhe,Su, Yeyu,Luo, Xiaozhou,Liu, Tao
supporting information, p. 10040 - 10048 (2021/03/26)
Incorporation of structurally novel noncanonical amino acids (ncAAs) into proteins is valuable for both scientific and biomedical applications. To expand the structural diversity of available ncAAs and to reduce the burden of chemically synthesizing them, we have developed a general and simple biosynthetic method for genetically encoding novel ncAAs into recombinant proteins by feeding cells with economical commercially available or synthetically accessible aromatic thiols. We demonstrate that nearly 50 ncAAs with a diverse array of structures can be biosynthesized from these simple small-molecule precursors by hijacking the cysteine biosynthetic enzymes, and the resulting ncAAs can subsequently be incorporated into proteins via an expanded genetic code. Moreover, we demonstrate that bioorthogonal reactive groups such as aromatic azides and aromatic ketones can be incorporated into green fluorescent protein or a therapeutic antibody with high yields, allowing for subsequent chemical conjugation.
Preparation method of alliin
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Paragraph 0063-0064; 0070-0071; 0077-0078; 0084-0085; 0092, (2020/07/08)
The invention discloses a preparation method of alliin. The preparation method comprises the following steps: in a microreactor, carrying out an oxidation reaction on deoxidized alliin represented bya formula 2 and an oxidizing agent in a solvent to obtain alliin represented by a formula 1, wherein the molar ratio of the oxidizing agent to the deoxidized alliin represented by the formula 2 is 0.9-1.2. The alliin preparation method has the advantages of high raw material conversion rate, high final product purity, high specific rotation, short reaction time, few side reactions, simple operation, simple post-treatment, and good application market.
Type of complex-BSA binding forces affected by different coordination modes of alliin in novel water-soluble ruthenium complexes
Zahirovi?, Adnan,?ili?, Dijana,Paveli?, Sandra Kraljevi?,Huki?, Mirsada,Muratovi?, Senada,Harej, Anja,Kahrovi?, Emira
, p. 5791 - 5804 (2019/04/17)
Three novel water-soluble ruthenium complexes having differently bound alliin ligands were prepared by solution synthesis and characterized by chemical analysis, and infrared, mass, nuclear magnetic resonance and electron paramagnetic resonance spectrosco
