500-85-6

Usage

Uses

Used in Chemical Analysis:
Indophenol is used as a spectrophotometric reagent for the determination of ammonia and paracetamol. Its blue color allows for the accurate measurement of these substances through the analysis of light absorption, providing a reliable method for detecting and quantifying their presence in samples.
Used in Environmental Testing:
In environmental science, Indophenol is utilized as an indicator in the Nessler method, a technique employed to measure the concentration of ammonia in water samples. This application is crucial for assessing water quality and ensuring the safety of aquatic ecosystems.
Used in Pharmaceutical Industry:
Within the pharmaceutical sector, Indophenol serves as a reagent in the analysis of paracetamol, a widely used medication. Its ability to react with and indicate the presence of this drug is essential for quality control and ensuring the efficacy and safety of pharmaceutical products.

InChI:InChI=1/C12H9NO2/c14-11-5-1-9(2-6-11)13-10-3-7-12(15)8-4-10/h1-8,14H

Upstream product

• 1752-24-5 4,4'-dihydroxydiphenylamine 
• 2150-58-5 phenol blue 
• 7761-80-0 indophenyl acetate 
• 42486-53-3 4-amino-2,6-dichlorophenol hydrochloride 
• 123-30-8 4-amino-phenol 
• 28739-71-1 2,6-dimethyl-3-acetylamino-phenol 
• 39495-15-3 4-acetamido-3-methylphenol 
• 123-31-9 hydroquinone 
• 1146-43-6 N-(4-hydroxyphenyl)-4-methylbenzenesulfonamide 
• 108-95-2 phenol 
• 71-43-2 benzene 
• 67-66-3 chloroform 
• 64-19-7 acetic acid 
• 51-78-5 p-aminophenol hydrochloride 

Downstream Products

• 77502-74-0 2,5-bis(4-hydroxyanilino)-1,4-benzoquinone 
• 123-30-8 4-amino-phenol 
• 123-31-9 hydroquinone 
• 1752-24-5 4,4'-dihydroxydiphenylamine 
• 7761-80-0 indophenyl acetate 
• 46700-12-3 N-oxyphenolindophenol 
• 132928-77-9 N-oxyphenolindophenyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside 
• 132928-75-7 N-oxyphenolindophenyl-β-D-glucopyranoside 

Relevant academic research and scientific papers

PURIFICATION AND PROPERTIES OF AN ESTERASE FROM CUCURBITA MAXIMA FRUIT TISSUE

An esterase from the fruit of Cucurbita maxima was purified to apparent homogeneity.The enzyme displays an Mr, of 36 000, a pI value of 4.9, a Stokes radius of 2.70 nm, diffusion coefficient of 9.25 x 1E-7 cm2/sec, possesses a homogeneous dimeric quaternary structure with a subunit Mr of 18 000.High esterolytic activity was observed with indophenyl acetate (1510 μmol/min/mg protein and p-nitrophenyl acetate (648 μmol/min/mg protein) while the enzyme displayed no carboxypeptidase, amidase, proteinase or aminopeptidase activities.Based on indophenyl acetate as substrate, the esterase has an optimum pH of 7.5 to 8.9 and a KM value.14 mM at pH 8.0.The esterolytic activity is strongly inhibited by mercaptide-forming and alkylating thiol reagents and by diisopropyl fluorophosphate and phenylmethylsulphonyl fluoride.Product inhibition by indophenyl was competitive (apparent Ki=90 μM) relative to indophenol acetate and parabolic.Acetate was without effect.Key Word Index - Cucurbita maxima; Cucurbitaceae; esterase.

Nitrite Reduction to Nitrous Oxide and Ammonia by TiO2 Electrons in a Colloid Solution via Consecutive One-Electron Transfer Reactions

The mechanism of nitrite reduction by excess electrons on TiO2 nanoparticles (eTiO2-) was studied under anaerobic conditions. TiO2 was loaded with up to 75 electrons per particle, induced by γ-irradiation of acidic TiO2 colloid solutions containing 2-propanol. Time-resolved kinetics and material analysis were performed, mostly at 1.66 g L-1 TiO2. At relatively low nitrite concentrations (R = [eTiO2-]o/[nitrite]o > 1.5), eTiO2- decays via two consecutive processes; at higher concentrations, only one decay step is observed. The stoichiometric ratio Δ[eTiO2-]/[nitrite]o of the faster process is about 2. This process involves the one-electron reduction of nitrite, forming the nitrite radical (k1 = (2.0 ± 0.2) × 106 M-1 s-1), which further reacts with eTiO2- (k2) in competition with its dehydration to nitric oxide (NO) (k3). The ratios k2/k3 = (3.0 ± 0.5) × 103 M-1 and k2 > 1 × 106 M-1 s-1 were derived from kinetic simulations and product analysis. The major product of this process is NO. The slower stage of the kinetics involves the reduction of NO by eTiO2-, and the detailed mechanism of this process has been discussed in our earlier publication. The results reported in this study suggest that several intermediates, including NO and NH2OH, are adsorbed on the titanium nanoparticles and give rise to inverse dependency of the respective reaction rates on the TiO2 concentration. It is demonstrated that the reduction of nitrite by eTiO2- yields mainly N2O and NH3 via consecutive one-electron transfer reactions.

Polyfluoroalloxy phosphonic and phosphinic acid derivatives: I. 1-Hydroxy-2,2,2-trichloroethylphosphinates

Polyfluoroalkyl esters of 1-hydroxy-2,2,2-trichloroethylphosphonic and aryl(alkyl-)phosphinic acids exhibited antienzyme activity towards esterases of animal and microbial origin. A good correlation is observed between high antiesterase activity of the target compounds and their physicochemical parameters, characterizing their structure.

Polyfluoroalkoxy phosphonic and phosphinic acid derivatives: II. Reversible esterase inhibitors

Polyfluoroalkyl esters of phosphoric, alkylphosphonic and 1-hydroxypolyfluoroalkylphosphonic acids exhibited significant antiesterase activity against various esterases of animal and microbial origin. Moreover, with some compounds reversible inhibition of enzymes was observed due to the specific influence of hydrophobic fragments of the target products.

Preparation of azomethines by reacting a 4,4-dihalo-5-pyrazolone with aromatic primary amines

4,4-Dihalo-5-pyrazolones react with unoxidized aromatic primary aromatic amines or alkyl or hydrazines to give azomethine dyes, azo dyes or hydrazones which are useful in photographic systems.

Diphenylamines for dyeing keratinous fibers

Diphenylamines having the formula SPC1 Wherein: Y represents a member selected from the group consisting of a hydroxy and amino, R1 and R3, each independently, represent a member selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, a ureido residue, and -NHCOR wherein R is lower alkyl, R2 represents a member selected from the group consisting of hydrogen, halogen, lower alkyl, lower alkoxy, a ureido residue, -NHCOR wherein R is lower alkyl, and --NHR8 wherein R8 represents a member selected from the group consisting of hydrogen, lower alkyl, lower hydroxyalkyl and carbamyl lower alkyl, with the proviso that when R2 is --NHR8, R3 is not hydrogen R4, R5, R6, and R7 each independently represent a member selected from the group consisting of hydrogen, halogen, lower alkyl and lower alkoxy, with the proviso that when Y is hydroxy, R2 is not hydrogen, halogen, lower alkyl or alkoxy and that when Y is amino, at least two of R1, R2 and R3 are other than a hydrogen and R 2 is not hydrogen when R4, R5, R6 and R7 are all hydrogen and the acid addition salts of said diphenylamines.