5934-29-2 Usage
Description
L-Histidine hydrochloride monohydrate is a white crystalline or granular powder that serves as a high-quality stabilizer, buffer, and salt in the pharmaceutical and biopharmaceutical industries. It plays a crucial role in the stabilization of biologic drugs during their manufacturing and formulation process, preventing aggregation and ensuring the quality and safety of the final product.
Uses
Used in Biomanufacturing:
L-Histidine hydrochloride monohydrate is used as a stabilizer, buffer, and salt in cell culture media formulations for biomanufacturing. It helps in the successful purification and formulation of biomolecules, particularly in high-risk applications where low bioburden and endotoxins are required.
Used in Cell Culture Research:
L-Histidine hydrochloride monohydrate is used in the study of human T-lymphoblastic leukemia cell line MOLT-4 to investigate the modulation of apoptosis, contributing to a better understanding of cell behavior and potential therapeutic interventions.
Used in Non-Animal Cell Culture Media:
L-Histidine monohydrochloride, derived from non-animal sources, is used in cell culture media formulations to avoid the risk of contaminating bioproducts with adventitious viruses, ensuring the safety and quality of the final product.
Used in Gluten-Induced Gastrointestinal Disease Treatment:
L-Histidine hydrochloride monohydrate can be used as an AhR activator to treat gluten-induced gastrointestinal diseases, providing a potential therapeutic option for patients suffering from these conditions.
Used in Hair Coloring Methods:
L-Histidine hydrochloride monohydrate is also utilized in the hair coloring industry, offering a high-quality and effective ingredient for hair color products.
General Information:
L-Histidine hydrochloride monohydrate is part of the Emprove Program, which provides extensive documentation to facilitate compliance with pharmaceutical and biopharma product regulations. The raw materials are offered with full supply chain transparency and risk mitigation, ensuring the highest quality and safety standards. The SAFC portfolio, which includes L-Histidine hydrochloride monohydrate, is produced according to applicable cGMP guidelines and undergoes strict quality control procedures.
Biochem/physiol Actions
L-Histidine is biosynthesized from ATP, 5-phosphoribosyl-1-pyrophosphate (PRPP), and glutamine. This essential amino acid can be degraded to glutamate by histidiase, urocanase and imidazolonepropionase; it is also the precursor of histamine by action of histidine decarboxylase.
Purification Methods
Crystallise the monohydrochloride from aqueous EtOH or 60% aqueous EtOH (m 259odec). Alternatively dissolve 10g in 50mL of H2O, decolourise with Norite, filter, evaporate it in a vacuum to a syrup, cool to room temperature, add 95% EtOH with stirring until slightly turbid, scratch the sides of the vessel until crystals form, then add slowly 40mL of EtOH and keep at 0o overnight, filter the solid off, wash it several times with EtOH and dry it in a vacuum. [Cox J Biol Chem 78 475 1929, Cox et al. J Biol Chem 81 73 1929, Greenstein & Winitz The Chemistry of the Amino Acids J. Wiley, Vol 3 pp 1972, 2098 1961, Beilstein 25 II 407, 25 III/IV 4346.]
Check Digit Verification of cas no
The CAS Registry Mumber 5934-29-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 5,9,3 and 4 respectively; the second part has 2 digits, 2 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 5934-29:
(6*5)+(5*9)+(4*3)+(3*4)+(2*2)+(1*9)=112
112 % 10 = 2
So 5934-29-2 is a valid CAS Registry Number.
InChI:InChI=1/C6H9N3O2.ClH.H2O/c7-5(6(10)11)1-4-2-8-3-9-4;;/h2-3,5H,1,7H2,(H,8,9)(H,10,11);1H;1H2/t5-;;/m0../s1
5934-29-2Relevant articles and documents
Investigations on the growth aspects and characterization of semiorganic nonlinear optical single crystals of l-histidine and its hydrochloride derivative
Anandan,Arivanandhan,Hayakawa,Rajan Babu,Jayavel,Ravi,Bhagavannarayana
, p. 508 - 513 (2014)
Semiorganic single crystals of l-histidine and l-histidine hydrochloride monohydrate have been obtained in a single solution prepared from the mixture of l-histidine and hydrochloric acid in 1:2 M ratio. Growth aspects of the single crystals have been discussed along with characterization studies. Crystal system and lattice parameters have been identified by X-ray diffraction analyses. It has been observed that the grown crystals possess orthorhombic system but with different set of lattice parameters. Presence of various functional groups has been identified and formation of two different crystals has been confirmed by Fourier transform infrared spectral analyses and FT-Raman studies. Linear and nonlinear optical properties have been studied by UV-Vis spectral analyses and Kurtz-Perry powder technique respectively. The thermal stability of the grown crystals was determined by thermal analyses. From the characterization studies it is found that both the crystals are useful for second harmonic generation applications.