6313-34-4

Upstream product

• 825-90-1 sodium p-hydroxybenzenesulfonate 
• 88-75-5 2-hydroxynitrobenzene 
• 4134-83-2 4-acetoxy-3-nitrobenzenesulfonate natrium salt 
• 74-89-5 methylamine 
• 62-53-3 aniline 
• 107-15-3 ethylenediamine 
• 753-90-2 trifluoroethylamine 
• 56-40-6 glycine 

Downstream Products

• 20075-38-1 3-Nitro-4-octanoyloxy-benzolsulfonsaeure 
• 19753-78-7 3-Nitro-4-acetoxy-benzol-sulfonsaeure 
• 112695-91-7 Z-Ala-OH 2-nitro-4-sulfenyl ester sodium salt 
• 4134-83-2 4-acetoxy-3-nitrobenzenesulfonate natrium salt 
• 70-54-2 2,6-diaminocaproic acid 

Relevant academic research and scientific papers

Sodium 4-Hydroxy-3-nitrobenzenesulfonate Trihydrate

The title compound, Na+*C6H4NO6S-*3H2O, crystallizes in double layers with the nearly planar organic anions oriented parallel to one another and the sulfonate groups directed towards the center of the sandwich, where they are bonded to Na+ cations.Each Na+ cation bonds to six O atoms in a distorted octahedral geometry.The coordination sphere contains two sulfonate O atoms, one nitro O atom from a different anion and three water molecules.There are hydrogen bonds involving the water molecules, the sulfonate O atoms and the hydroxyl groups.

A minimalistic hydrolase based on co-assembled cyclic dipeptides

The self-assembly of small peptides into larger aggregates is an important process for the fundamental understanding of abiogenesis. In this article we demonstrate that blends of cyclic dipeptides (2,5-diketopiperazines-DKPs) bearing either histidine or c

Method for preparing sodium 3-nitrobenzene sulfonate and derivative thereof through chlorosulfonic acid sulfonation

The invention discloses a method for preparing sodium 3-nitrobenzene sulfonate and a derivative thereof through chlorosulfonic acid sulfonation. The method comprises the following steps: 1) putting anitrobenzene raw material and chlorosulfonic acid into a reaction container, heating to 90-150 DEG C, keeping the temperature, carrying out a sulfonation reaction, and discharging a hydrogen chloridegas generated in the reaction process outside the reaction system in time, wherein the mole ratio of the nitrobenzene raw material to the chlorosulfonic acid is 1:(0.4-0.6); 2) carrying out aftertreatment on the reaction liquid obtained in the step 1) by using soda, thereby obtaining the sodium 3-nitrobenzene sulfonate or the derivative thereof. By adopting the method, a reverse thinking mode is adopted, the amount of the chlorosulfonic acid is reduced till being smaller than that of the nitrobenzene, and then the small amount of the chlorosulfonic acid can be converted by using the excessiveamount of the nitrobenzene; therefore, the method disclosed by the invention has no problem of treating residual chlorosulfonic acid; in addition, the excessive nitrobenzene can be easily recycled andcirculated, the hydrogen chloride as a byproduct can be also easily separated and recycled.

Method of conjugating an activated ester to an amine-containing biological material

A coupling agent which is an activated ester such as N-maleimido-6-aminocaproyl-HNSA (mal-sac-HNSA) is formed by reacting 4-hydroxyl-3-nitrobenzene sulfonic acid sodium salt (HNSA) with a carboxylic acid moiety of a compound such as N-maleimido-6-aminocaproic acid. The coupling agent is reacted with an amino group of an amine-containing biological material such as a protein at a pH of about 5.5 to 10.0 and HNSA is released. The released HNSA is spectroscopically measured at a wavelength of from about 350 nm to about 500 nm to precisely monitor and control conjugating of the coupling agent to the biological material. The resulting product is coupled to a sulfhydryl group or other group of another material to provide cross-linking between the two. This enables joining the biological material to one another, to a support matrix, to a label, to a hapten, and to other materials. An immunotoxin for therapeutic use can be prepared by conjugating the coupling agent to an antibody such as a monoclonal antibody with tumor cell specificity and then joining the resultant conjugate to a cytotoxic molecule.

Kinetics and mechanism of aminolysis of phenyl acetates in aqueous solutions of poly(ethylenimine)

Second-order rate constants (kn) for the aminolysis of some phenyl acetates with poly(ethylenimine) (PEI) were obtained in a pH range 4.36-11.20 at 25 °C in 1 M KC1. Linear Bronsted-type plots (log kn vs pKN of PEI) were found for less reactive esters 2-nitrophenyl acetate, 4-acetoxy-3-chlorobenzoic acid, and 4-acetoxybenzenesulfonate with slopes of 0.92, 0.99, and 0.82, respectively. Curved plots were obtained for 3-acetoxy-2,6-dinitrobenzoic acid and 4-acetoxy-3-nitrobenzene-sulfonate, which are consistent with a stepwise reaction. The most likely mechanism involves the existence of a tetrahedral intermediate (T±) and a change in the rate-determining step from its breakdown to its formation when the basicity of the polyamine increases. A semiempirical equation was used to calculate the values of limiting slopes of the plots (0.9 and 0.1 for both esters) and pKN at the center of the curvature of the plots (p-KN° = 7.94 and 9.02, respectively). The values of pKN° are lower than those estimated for the aminolysis of the same esters with simple monomeric amines (pKn° > 11) because of a better leaving ability of the aryl oxide ion from the tetrahedral intermediate when amino groups of PEI instead of simple amines are involved. Estimation of the pK's of the reactive intermediates and of the microscopic rate constants for the proton transfer from T± to PEI or from PEIH+ to T± indicates that either base or acid catalysis is unimportant in the aminolysis of these esters by PEI.

Certain pyridyl-di-sulfide-alkylenecarbonxylate-ortho-nitro-phenylsulfonic acid esters useful for coupling biological materials

Coupling of biological materials is disclosed via 4-hydroxy-3-nitrobenzene sulfonic acid sodium salt (HNSA) esters. The novel activated esters are structured so as to react at one end with an amine group of a selected biological or non-biological material, thereby releasing the spectroscopically monitorable HNSA dianion, and at the other end with, typically, a sulfhydryl group of a second material which may or may not be biological. The esters provide for a cross-linking reaction that may be easily controlled and monitored.

Certain maleimide-N-alkylenecarboxylate-ortho-nitrobenzenesulfonic acid esters and derivatives useful for coupling biological materials

Coupling of biological materials is disclosed via 4-hydroxy-3-nitrobenzene sulfonic acid sodium salt (HNSA) esters. The novel activated esters are structured so as to react at one end with an amine group of a selected biological or non-biological material, thereby releasing the spectroscopically monitorable HNSA dianion, and at the other end with, typically, a sulfhydryl group of a second material which may or may not be biological. The esters provide for a cross-linking reaction that may be easily controlled and monitored.