The main Analysis method for Chrysanthemin is high performance liquid chromatography (HPLC). Chromatographic analysis condition is commonly as the following: chromatographic column: Phe nomenex Luna Su C18 (250mm×4.60mm，5μm)； mobile phase: phase A is 0.5 % Phosphoric acid solution，phase B is water-acetonitrile (50: 50，V/ V). gradient elution：0min(18%B),23min(50%B),28min(18%B),33min(18%B),40min(stop)；flow velocity：0.8mL/min；Detection wavelength：520nm；column temperature：30℃. Figure 1 shows the high performance liquid chromatogram (HPLC) figure of cyanidin-3-O-glucoside(Chrysanthemin) in mulberry extracting solution.
It is reported that long time high temperature and light has an obvious destructive effect on Mulberry Anthocyanins. At room temperature, the degradation rate of mulberry Chrysanthemin increased significantly with the extension of storage time. In long-term exposure to air, the light might induce the degradation of Chrysanthemin. The specific reasons need to be further studied. Therefore, anthocyanin extraction should be carried out in time after picking mulberry fruit. In addition fresh mulberry fruit should be stored in a cool, well-ventilated warehouse.
Anthocyanin also called anthocyanidin, is the main color substances in plant flowers and fruit. It is honored as the 7th essential nutrients of the human body after water, protein, fat, carbohydrates, Vitamins and minerals. Thus, it becomes the most perspective natural pigment. Mulberry anthocyanins has been approved by the Ministry of public health as a kind of food additive and widely used in food and health care products. Cyanidin-3-O-glucoside is one of the main ingredients of mulberry anthocyanins. Black soy bean is a traditional kind of crops in our country. It has a long food and medical history. Black soybean has been used for enriching the blood, activating the blood and Ufa beauty since the ancient times. Black bean peel, also called black clothes is the dry and mature skin of black soybean which is a kind of leguminous plants. It is rich in anthocyanins which mainly consist of delphinium and cyaniding. It has lots of healthgiving benefits such as antioxidant activity, Inhibiting mutagenesis, anti-inflammatory, prevention of cancer, anti-aging, etc. It has been a raw material of natural food additives and healthcare food.
Until now, only Korean scholar Lee reported 5 kinds of anthocyanins separated from black bean peel as the following: Cyanidin-3-O-glucoside,delphinidin-3-O-glucoside, petunidin-3-O-glucoside, malvidin-3-O-glucoside and Cyanidin. In addition, some research papers reported that Cyanidin-3-O-glucoside is an important ingredient of black bean peel.
Anthocyanin, as a kind of flavonoid plant chemicals, occurs ubiquitously in pants. Anthocyanins extracted from food or plants often contain different anthocyanins such as cyaniding, pelargonidin, petunidin, malvidin, etc. Glucoside can also be divided into single, double, three glucoside structures and anthocyanins with different structure have different biological activities.
Anthocyanin has many therapeutic effects such as antioxidant, Anti-atherosclerosis, adjusting blood fat, etc. cyanidin-3-O-β-glucoside is one of the main ingredients of Anthocyanin.
A new study claims that purple corn anthocyanin can inhibit the body weight growth of high-fat induced mice. But its effective components and the mechanism is not clear. It is speculated that the 7% content of Chrysanthemin in purple corn anthocyanins played a leading role.
Cyanidol 3-Glucoside is a anthocyanin that is naturally occurring in various fruits, vegetable and plants. Cyanidol 3-Glucoside is known to exhibit high antioxidant capacity and neuroprotective effect
s by triggering mobilization of cellular free sialic acid and utilizing it as an additional biological antioxidant in brain neural cells.
Modern pharmacology study indicated that C3G has many significant medical and health care effects such as antioxidant, anti-tumor, protecting the nervous system, recovering Transient visual impairment, etc. In addition, studies indicated that Chrysanthemin can be used as protective agent for protecting cotton leaves against cotton bollworm.
C3G in Black bean peel can reduce the production of reactive oxygen species in 3T3-L1fat cells, thus, Dose dependently improved the resistance of H2O2 or TNF-α induced insulin. At the same time, it can reduce the expression of retinol-binding protein in type II diabetic rats, thus, improve hyperglycemia and insulin sensitivity and the symptoms of diabetes.