91-95-2 Usage
Description
3,3'-Diaminobenzidine (DAB) is a chemical compound that serves as a precursor to polybenzimidazole. It is widely recognized for its role in immunohistochemical staining of nucleic acids and proteins. DAB's unique property of being oxidized by hydrogen peroxide in the presence of hemoglobin to produce a dark-brown color makes it a valuable tool in various applications.
Uses
Used in Forensic Science:
3,3'-Diaminobenzidine is used as a reagent for detecting fingerprints in blood. Its ability to create a dark-brown color upon oxidation by hydrogen peroxide in the presence of hemoglobin makes it an effective tool for forensic analysis.
Used in Immunohistochemistry:
3,3'-Diaminobenzidine is used as a peroxidase substrate in the immunohistochemical staining of nucleic acids and proteins. This application is crucial for the visualization and identification of specific biological molecules within tissue samples.
Used in Analytical Chemistry:
3,3'-Diaminobenzidine is used as a reagent for the spectrophotometric determination of selenium. This use highlights its versatility in analytical processes, contributing to the accurate measurement of trace elements.
Used in the Synthesis of Coordination Polymers:
3,3'-Diaminobenzidine may be used for the synthesis of a useful, linear, Schiff-base coordination polymer. This application showcases its potential in the development of new materials with specific properties and functions.
Used in Medical Research:
As a dark brown dye, 3,3'-Diaminobenzidine has been used as an antibody-specific stain to identify paired antibodies in breast tissue. This application aids in the study of antibody distribution and interactions within tissues, contributing to a better understanding of immune responses and disease mechanisms.
Synthesis
3,3'-Diaminobenzidine can be synthesized by treating 3, 3'-dichlorobenzidine with ammonia in the presence of copper catalyst at high temperature and pressure.
Biochem/physiol Actions
DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.
Safety Profile
Suspected carcinogen withexperimental tumorigenic data. Moderately toxic byingestion. Mutation data reported. When heated todecomposition it emits toxic fumes of NOx.
References
3,3′-Diaminobenzidine staining interferes with PCR-based DNA analysisDOI:10.1038/s41598-018-19745-9Transition metal complexes of novel binuclear Schiff base derived from 3,3′-diaminobenzidine: synthesis, characterization, thermal behavior, DFT, antimicrobial and molecular docking studiesDOI:10.1080/00958972.2020.17523723, 3′-Diaminobenzidine with dual o-phenylenediamine groups: two in one enables visual colorimetric detection of nitric oxideDOI:10.1007/s00216-020-02482-2A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.DOI:10.1016/0003-2697(73)90144-9
Check Digit Verification of cas no
The CAS Registry Mumber 91-95-2 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 9 and 1 respectively; the second part has 2 digits, 9 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 91-95:
(4*9)+(3*1)+(2*9)+(1*5)=62
62 % 10 = 2
So 91-95-2 is a valid CAS Registry Number.
91-95-2Relevant articles and documents
Synthesis method of benzidine compound
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, (2018/07/30)
The invention discloses a synthesis method of a benzidine compound. A compound (I) is subjected to catalytic coupling reaction to obtain a compound (II); the compound (II) is subjected to alkaline oracidic hydrolysis reaction process to obtain a compound (III); the compound (III) is subjected to catalytic hydrogenation reduction reaction in an organic system to obtain a benzidine compound (V); orthe compound (II) is subjected to catalytic hydrogenation reduction reaction in an organic solvent to obtain a compound (V); the compound (V) is subjected to alkaline or acidic hydrolysis reaction toobtain a compound (IV). The method disclosed by the invention overcomes various defects of the existing method; the conventional commercial catalysts are used; the reaction time is greatly shortened;the capacity is improved. Noteworthily, the organic solvent can be recovered and reused; hydroiodic acid or hydrobromide generated through coupling reaction can be smoothly converted into iodides orbromide salts with high economic values through treatment. Therefore, the method disclosed by the invention is an economic easy-to-industrialize green method.
METHOD OF MANUFACTURING 3, 3' , 4, 4'-TETRAAMINOBIPHENYL
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Page/Page column 5-6, (2009/06/27)
An object of the present invention is to provide an efficient method of manufacturing 3,3′,4,4′-tetraaminobiphenyl with a smaller number of steps. The manufacturing method of 3,3′,4,4′-tetraaminobiphenyl includes reacting the amino groups of a 4-halo-o-phenylenediamine with an inorganic sulfur compound to lead to a 5-halo-2,1,3-benzothiadiazole, subsequently coupling two molecules of the benzothiadiazole together to form a 5,5′-bis(2,1,3-benzothiadiazole) and then deprotecting the amino groups to yield 3,3′,4,4′-tetraaminobiphenyl.
