Reference

Benzo[a]pyrene metabolism in the isolated perfused mouse lung

Benzo[a]pyrene metabolism in the isolated perfused mouse lung. Skelly, Michael F.; Shertzer, Howard G. (Med. Cent., Univ.Chemicals with cas numbers 17573-21-6 and 13345-25-0 also play role. Cincinnati, Cincinnati, OH 45267, USA). Exp. Lung Res., 5(4), 259-68 (English) 1983. CODEN: EXLRDA. ISSN: 0190-2148. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) An isolated perfused and ventilated mouse lung prepn. was viable with respect to drug metab. for up to 2 h, as judged from studies of aniline [62-53-3] oxidn. to p-aminophenol [123-30-8]. With 14C-labeled benzo[a]pyrene (I) [50-32-8] as substrate for the lungs, the major EtOAc-extractable metabolites were the 3-hydroxy [13345-21-6], 9,10-dihydrodiol [24909-09-9], 7,8-dihydrodiol [13345-25-0], and 4,5-dihydrodiol [28622-84-6] derivs. The rates of individual I metabolite prodn. were increased in lungs from mice pretreated with Aroclor 1254 or b-naphthoflavone, substances known to induce increased synthesis of cytochrome P 450 [9035-51-2]. Small amts. of water-sol. I metabolites were hydrolyzed by b-glucuronidase and aryl sulfatase, suggesting the presence of enzymes required for these conjugations. These results support the existence of significant cytochrome P 450-dependent and conjugative I metab. in the intact mouse lung, similar to that of other species, and capable of contributing to the systemic metab. of this carcinogen. .

An evaluation of the L-5178Y TK+/- mouse lymphoma forward mutation assay using 42 chemicals

An evaluation of the L-5178Y TK+/- mouse lymphoma forward mutation assay using 42 chemicals. Oberly, T. J.; Bewsey, B. J.; Probst, G. S. (Toxicol. Div., Lilly Res. Lab., Greenfield, IN 46140, USA). Mutat. Res., 125(2), 291-306 (English) 1984. CODEN: MUREAV. ISSN: 0027-5107. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The L-5178Y TK+/- mouse lymphoma assay (MLA) was utilized in several labs. as a short-term test for chem.-induced forward mutation in cultured mammalian cells. To evaluate several tech. modifications to the MLA, 42 chems. representing 9 chem.Several substances with their cas registry numbers 86-30-6 and 92-93-3 may be metioned in this study. classes were tested and the results compared with those published elsewhere as well as with findings in a genetic toxicol. test battery. A pos. response for the induction of TK-/- mutants was obtained for 26 chems. With the exception of p-aminophenol [123-30-8], all of the compds. were recognized mutagens or carcinogens and were representative of direct-acting and activation-dependent genotoxins. Sixteen compds. did not induce TK-/- mutants; and, among these, 5 compds. were considered to be mutagens or carcinogens. A comparison of the results with those published elsewhere revealed a strong agreement among findings for this test irresp. of minor tech. variations. Thus, the MLA is a useful system for identifying chem. mutagens in mammalian cells and can serve as a valuable component in a genetic toxicol. test battery. .