Reference

Determination of 2-phenylethylamine in rat brain after MAO inhibitors, and in human CSF and urine by capillary GC and chemical ionization MS

Determination of 2-phenylethylamine in rat brain after MAO inhibitors, and in human CSF and urine by capillary GC and chemical ionization MS. Lauber, J.; Waldmeier, P. C. (Res. Dep., Ciba-Geigy Ltd., Basle CH-4002, Switz.). J. Neural Transm., 60(3-4), 247-64 (English) 1984. CODEN: JNTMAH. ISSN: 0300-9564. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A highly specific and sensitive method for the detn. of b-phenylethylamine (PEA) [64-04-0] in biol. material is presented. It involves prepurifn. of the exts. on Sep-Pak C18 cartridges, derivatization with heptafluorobutyric acid anhydride, gas chromatog. on 50 m capillary columns and quantification by chem. ionization mass spectrometry. Using this method, levels of PEA in the rat brain and the effects of various MAO [9001-66-5] inhibitors on PEA were detd. PEA controls levels were found to vary considerably: the lowest and highest values found were 0.4 and 12.5 ng/g tissue (n = 25). Within one and the same control group, the variation was somewhat smaller. The preferential or specific inhibitors of MAO A, amiflamine [77518-07-1], cimoxatone [73815-11-9], CGP 11305A [63638-90-4], moclobemide [71320-77-9] and toloxatone [29218-27-7] did not alter rat brain PEA even at high doses. In contrast, the preferential inhibitors of MAO B, deprenil [2323-36-6], pargyline [555-57-7] and MD 780236 [84269-97-6], as well as the nonselective agent tranylcypromine [155-09-9], caused strong (up to about 60-fold) increases. The threshold doses corresponded to those which caused about an 80% inhibition of MAO B as measured ex vivo. The method was also used to det. the concn. of PEA in human cerebrospinal fluid and urine.

Discrimination of the amphetamine cue

Discrimination of the amphetamine cue. Effects of A, B and mixed type inhibitors of monoamine oxidase. Porsolt, R. D.; Pawelec, Christiane; Roux, Sylvain; Jalfre, M. (Cent. Rech. Delalande, Rueil-Malmaison 92500, Fr.). Neuropharmacology, 23(5), 569-73 (English) 1984. CODEN: NEPHBW. ISSN: 0028-3908. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Rats were trained to discriminate between the effects of 0.6 mg/kg of (+)-amphetamine [51-64-9] given i.p. and saline in a 2-lever operant task, according to a fixed ratio (FR10) schedule of food reinforcement. Once trained (>90% responding on the appropriate lever during sessions with drug and saline, resp. over 2 wk), they were given tests of generalization with various type A, type B or mixed type monoamine oxidase [9001-66-5] inhibitors (MAOI). Generalization was tested during the 1st 100 s of a 15 min trial during which no reinforcements were given (extinction) followed by reinforcement on both levers for the rest of the session. Generalization to amphetamine was estd. using both the percentage of responding on the "drug lever" and a measure of choice of lever. None of the type A MAOI's tested [MD 780515 [73815-11-9], clorgyline [17780-72-2], LY 51641 [5388-85-2], Ro 11-1163 [71320-77-9], toloxatone [29218-27-7]] showed generalization towards the lever for amphetamine. Clear or partial amphetamine-like responding was obsd. with the B type MAO (±)-deprenyl [4530-70-5] and LY 54761 [90832-33-0] but not with 2 other type B MAOI's MD 240928 [84145-89-1] or Pargyline [555-57-7]. Tranylcypromine [155-09-9], but not nialamide [51-12-7] (mixed type MAOI's), induced dose-dependent responding on the lever for amphetamine. Thus, amphetamine-like activity was not an intrinsic property of A or B type MAOI's.