Reference

Mechanism of oxidative lysis and lipid peroxidation of vitamin E-deficient erythrocytes

Mechanism of oxidative lysis and lipid peroxidation of vitamin E-deficient erythrocytes. Krishnamurthy, S.; Bai, N. Jayanthi; George, Thomas (Dep. Biochem., Med. Coll., Trivandrum, India). Indian J. Biochem. Biophys., 21(6), 361-4 (English) 1984. CODEN: IJBBBQ. ISSN: 0301-1208. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Effects of synthetic antioxidants, hydroxyl and singlet O radical scavengers, catalase [9001-05-2], superoxide dismutase [9054-89-1], and of EDTA (an inhibitor of lipid peroxidn.) on the oxidative lysis and stromal lipid peroxidn., of vitamin E [1406-18-4]-deficient rat erythrocytes were studied. Among the antioxidants studied, retinol [68-26-8], butylated hydroxy toluene (BHT) [128-37-0] and santoquin [91-53-2] enhanced the lysis, but inhibited the peroxidn; while N,N'-diphenyl-p-phenylenediamine (DPPD) [74-31-7], 2,5-bis-1-1-dimethylpropyl hydroquinone (DAH) [79-74-3] and a-tocopherol inhibited both. Hydroxyl radical scavengers were effective in inhibiting both lysis and peroxidn.; singlet O radical scavengers were without effect on both phenomena. EDTA inhibited the peroxidn., but also showed a definite effect on the lysis. Beef liver catalase inhibited both lysis and lipid peroxidn., and bovine erythrocyte superoxide dismutase showed no effect. The osmotic fragility of erythrocytes was not affect in vitamin E deficiency. It is concluded that the oxidative lysis of vitamin E-deficient erythrocytes is a result of stromal lipid peroxidn., initiated by hydroxyl radicals, generated from H2O2.

Effect of vitamins, antioxidants and sulfhydryl compounds on in vitro rat brain lipid peroxidation

Effect of vitamins, antioxidants and sulfhydryl compounds on in vitro rat brain lipid peroxidation. Kartha, V. N. R.; Krishnamurthy, S. (Dep. Biochem., T. D. Med. Coll., Alleppey, India). Int. J. Vitam. Nutr. Res., 48(1), 38-43 (English) 1978. CODEN: IJVNAP. ISSN: 0300-9831. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Section cross-reference(s): 3 The effect of retinol [68-26-8], retinyl acetate [127-47-9], g-tocopherol [7616-22-0], a-tocopheryl acetate [58-95-7], synthetic antioxidants (DPPD [74-31-7], BHT [128-37-0], DAH [79-74-3], and Ethoxyquin [91-53-2]), and SH compds. (reduced glutathione [70-18-8] and cysteine [52-90-4]) on rat brain lipid peroxidn. was studied by using the thiobarbituric acid method. Retinol and retinyl acetate inhibited brain lipid peroxidn., a-tocopherol was less effective than vitamin A, and a-tocopheryl acetate had no antioxidant activity. Ascorbic acid [50-81-7] stimulated peroxidn. at pH 5.0. The synthetic antioxidants were all potent inhibitors of brain lipid peroxidn., DPPD being the most effective; whereas the SH compds. at 10-3M had a slight potentiating effect.