10191-18-1 Usage
Description
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.The effect of BES and other zwitterionic buffers on the bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.
Uses
Different sources of media describe the Uses of 10191-18-1 differently. You can refer to the following data:
1. BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C. Absorbance (100 mM, 260 nm):≤0.05.BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
2. BES is used as a buffer to maintain the pH of the solutions in biological experiments. It is useful for diagnostic assay manufacturing industry. It induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. It acts as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
3. BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Application
BES, or N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, can be used to study molecular biology, biological buffers, molecular biology reagents and zwitterionic compounds. BES has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES has also been used to determine that saline and buffers minimize the action of interfering factors in the bacterial endotoxins test.BES is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
General Description
BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Flammability and Explosibility
Notclassified
Purification Methods
Crystallise BES from aqueous EtOH. [Beilstein 4 IV 3290.]
Check Digit Verification of cas no
The CAS Registry Mumber 10191-18-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,1,9 and 1 respectively; the second part has 2 digits, 1 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 10191-18:
(7*1)+(6*0)+(5*1)+(4*9)+(3*1)+(2*1)+(1*8)=61
61 % 10 = 1
So 10191-18-1 is a valid CAS Registry Number.
InChI:InChI=1/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
10191-18-1Relevant articles and documents
Hydrogen ion buffers for biological research.
Good,Winget,Winter,Connolly,Izawa,Singh
, p. 467 - 477 (1966)
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A new strategy for the synthesis of taurine derivatives using the 'safety-catch' principle for the protection of sulfonic acids
Seeberger, Sonja,Griffin, Roger J.,Hardcastle, Ian R.,Golding, Bernard T.
, p. 132 - 138 (2008/03/14)
The safety-catch principle has been applied for the development of a new method for protecting sulfonic acids. 2,2-Dimethylsuccinic acid was reduced to 2,2-dimethylbutane-1,4-diol, which was selectively silylated to give 4-(tert-butyldiphenylsilanyloxy)-2,2-dimethylbutan-1-ol. Reaction of the latter compound with 2-chloroethanesulfonyl chloride in the presence of triethylamine afforded 4-(tert-butyldiphenylsilyloxy)-2,2-dimethylbutyl ethenesulfonate directly. The ethenesulfonate underwent Michael-type addition with secondary amines to give protected derivatives of taurine (2-aminoethanesulfonic acid). Deprotection was achieved on treatment with tetrabutylammonium fluoride, whereby cleavage of the silicon-oxygen bond led to an intermediate alkoxide that immediately cyclised to 2,2-dimethyltetrahydrofuran with liberation of a sulfonate. Pure sulfonic acids were obtained from the crude product by ion exchange chromatography on a strongly basic resin, which was eluted with aqueous acetic acid. The method developed should be generally applicable to the protection of sulfonic acids and is amenable to a multiparallel format. This journal is The Royal Society of Chemistry.
Isolation of nucleic acids
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, (2008/06/13)
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
