77-86-1Relevant articles and documents
Thermodynamics of the hydrolysis of TRISH+ in (water + 10 or + 20 mass per cent of acetonitrile) from 263.15 to 298.15 K
Vega, Carmen A.,Vila, Pablo
, p. 385 - 390 (1987)
The standard potentials of (AgCl + 1/2H2 = Ag + HCl) in (water + 10 or + 20 mass per cent of acetonitrile) were used to determine the acidity constants of protonated tris(hydroxymethyl)aminomethane (TRISH+).Measurements were made at eight temperatures ranging from 263.15 to 298.15 K at 5 K intervals in cells without liquid junction.The molalities of TRISH+ ranged from 0.1 to 0.005 mol * kg-1.The standard molar Gibbs free energy change ΔrGm0 of the reaction was calculated.From the values obtained in the literature for water and our values for the mixed solvents, the standard molar Gibbs free energy of transfer ΔtGm0 was also estimated.It was found that the hydrolysis of TRISH+ is less favored in the mixtures than in pure water.
Sanger dideoxy sequencing of DNA
Walker, Sarah E.,Lorsch, Jon
, p. 171 - 184 (2013)
While the ease and reduced cost of automated DNA sequencing has largely obviated the need for manual dideoxy sequencing for routine purposes, specific applications require manual DNA sequencing. For instance, in studies of enzymes or proteins that bind or modify DNA, a DNA ladder is often used to map the site at which an enzyme is bound or a modification occurs. In these cases, the Sanger method for dideoxy sequencing provides a rapid and facile method for producing a labeled DNA ladder.
COMPOSITION FOR STERILIZATION AND CLEANING, METHOD FOR PRODUCING SAME, AND STERILIZATION AND CLEANING METHOD EMPLOYING SAME
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, (2022/01/08)
A composition for sterilization and cleaning which comprises 0.001-30 mass % peracetic acid, 0.001-30 mass % hydrogen peroxide, 1-70 mass % acetic acid and/or salt thereof, 0.001-5 mass % polyoxyethylene lauryl ether acetate or salt thereof, and water, the composition having a pH of 4.0-10.0.
Solution Dynamics of Hybrid Anderson-Evans Polyoxometalates
Salazar Marcano, David E.,Lentink, Sarah,Moussawi, Mhamad A.,Parac-Vogt, Tatjana N.
supporting information, p. 10215 - 10226 (2021/05/31)
Understanding the stability and speciation of metal-oxo clusters in solution is essential for many of their applications in different areas. In particular, hybrid organic-inorganic polyoxometalates (HPOMs) have been attracting increasing attention as they combine the complementary properties of organic ligands and metal-oxygen nanoclusters. Nevertheless, the speciation and solution behavior of HPOMs have been scarcely investigated. Hence, in this work, a series of HPOMs based on the archetypical Anderson-Evans structure, δ-[MnMo6O18{(OCH2)3C-R}2]3-, with different functional groups (R = -NH2, -CH3, -NHCOCH2Cl, -NCH(2-C5H4N) {pyridine; -Pyr}, and -NHCOC9H15N2OS {biotin; -Biot}) and countercations (tetrabutylammonium {TBA}, Li, Na, and K) were synthesized, and their solution behavior was studied in detail. In aqueous solutions, decomposition of HPOMs into the free organic ligand, [MoO4]2-, and free Mn3+ was observed over time and was shown to be highly dependent on the pH, temperature, and nature of the ligand functional group but largely independent of ionic strength or the nature of the countercation. Furthermore, hydrolysis of the amide and imine bonds often present in postfunctionalized HPOMs was also observed. Hence, HPOMs were shown to exhibit highly dynamic behavior in solution, which needs to be carefully considered when designing HPOMs, particularly for biological applications.
PROTEIN STERILISATION BY RADIATION AND ADDITION OF A STABILISING COMPOSITION
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, (2010/03/02)
A method of sterilising a protein, comprises exposing to ionising radiation an at least substantially dry composition comprises a protein and a protective compound or combination of protective compounds having both of the following characteristics: (i) a rate of reaction with singlet oxygen greater than 1×10 7 L mol?1 S?1; (ii) being a reducing agent whilst at the same time containing a proton dissociable group with a pKa no more than 3 units from the pH of the composition. The compound having characteristic (i) is selected from histidine, thiamine and tryptophan, the compound having characteristic (ii) is selected from methionine, malate, citrate, lactate and tiron. The radiation is gamma radiation or electron beam, whereby the preferred dose is 15-40 kGy.
Topical acne vulgaris composition with a sunscreen
-
, (2009/03/07)
Increased compliance in the use of topical sunscreens is obtained by combining a topical sunscreen agent in a formulation containing an antibacterial medication such as azelaic acid or an antibiotic.
Topical acne vulgairs medication with a sunscreen
-
, (2008/06/13)
Increased compliance in the use of topical sunscreens is obtained by combining a topical sunscreen agent in a formulation containing an antibacterial medication such as azelaic acid or an antibiotic.
The multiple Maillard reactions of ribose and deoxyribose sugars and sugar phosphates
Munanairi, Admire,O'Banion, Steven K.,Gamble, Ryan,Breuer, Elizabeth,Harris, Andrew W.,Sandwick, Roger K.
, p. 2575 - 2592 (2008/03/27)
Ribose 5-phosphate (R5P) undergoes the Maillard reaction with amines at significantly higher rates than most other sugars and sugar phosphates. The presence of an intramolecular phosphate group, which catalyzes the early stages of the Maillard reaction, provides the opportunity for the R5P molecule to undergo novel reaction paths creating unique Maillard products. The initial set of reactions leading to an Amadori product (phosphorylated) and to an α-dicarbonyl phosphate compound follows a typical Maillard reaction sequence, but an observed phosphate hydrolysis accompanying the reaction adds to the complexity of the products formed. The reaction rate for the loss of R5P is partially dependent on the pKa of the amine but also is correlated to the protonation of an early intermediate of the reaction sequence. In the presence of oxygen, a carboxymethyl group conjugated to the amine is a major product of the reaction of R5P with N-acetyllysine while little of this product is generated in the absence of oxygen. Despite lacking a critical hydroxyl group necessary for the Maillard reaction, 2-deoxyribose 5-phosphate (dR5P) still generates an Amadori-like product (with a carbonyl on the C-3 carbon) and undergoes phosphate cleavage. Two highly UV-absorbing products of dR5P were amine derivatives of 5-methylene-2-pyrrolone and 2-formylpyrrole. The reaction of dR5P with certain amines generates a set of products that exhibit an interesting absorbance at 340 nm and a high fluorescence.
Structure-stability correlations for imine formation in aqueous solution
Godoy-Alcantar,Yatsimirsky, Anatoly K.,Lehn
, p. 979 - 985 (2007/10/03)
Imine formation between 25 aldehydes and 13 amines in aqueous solution in the pH range 7-11 was studied by 1H NMR spectroscopy. A three-parameter linear equation correlating logarithms of imine formation constants with pKa and HOMO energies of amines and LUMO energies of aldehydes is proposed. In view of the widespread occurrence of imine-forming processes in both chemistry and biology, the data presented are of significance for physical organic chemistry and of particular interest for dynamic combinatorial chemistry. Copyright
Compositions of matter having bioactive properties
-
, (2008/06/13)
Particles of coordinated complex comprising a basic, hydrous polymer and a capacitance adding compound, as well as methods for their production, are described. These complexes exhibit a high degree of bioactivity making them suitable for a broad range of applications through their incorporation into conventional vehicles benefiting from antimicrobial and similar properties.
