- Purification of Chlorophyll α
-
Forms green crystals from Me2CO, Et2O + H2O, Et2O + hexane + H2O or Et2O +pentane + H2O. It is sparingly soluble in MeOH and insol in pet ether. In alkaline soln it gives a blue-green colour with deep red fluorescence. A very crude mixture has been purified by chromatography on low melting polyethylene (MI 0.044; 'Dow' melting index MI < 2) and developed with 70% aq Me2CO. The order of effluent from the bottom of the column is: xanthophylls, chlorophyll β, chlorophyll α, phaeophytins and carotenes. A mixture of Chlorophyll α (CAS NO. ) and β is best separated by chromatography on sugar and the order is chlorophyll β elutes first followed by chlorophyll α. To an Me2CO-H2O soln of chlorophylls 200mL of iso-octane is added and the mixt shaken in a separating funnel and the H2O is carefully removed. The iso-octane layer is dried (Na2SO4) and applied to a glass column (5cm diameter) dry packed with l000mL of powdered sucrose which has been washed with 250mL of iso-octane. Elution with 0.5% of isopropanol in iso-octane gives chlorophyll α. Keeping the eluate overnight at 0°C yields micro crystals which are collected by filtration or centrifugation (Yield 40mg).
Prev:No record
Next:No record - 【Back】【Close 】【Print】【Add to favorite 】
- Related information
- One Synthetic Approach of α-Naphthyl isothiocyanate
- One Synthetic Approach of ar-Tetrahydro-α-Naphthol
- Preparation of (-)-α-Pinene
- Synthesis of 1,3,4,6-Tetra-O-acetyl-2-deoxy-α-D-glucopyranose
- Production Method of α,α-Difluorotoluene and Benzenesulfinyl fluoride
- Preparation of 2-Phenylperfluoropropene
- Synthesis of α-Aminodiethylacetic acid
- Synthesis of α-Methyl mannoside
- Synthesis of α-Chloroanthraquinone
- Production Method of Potassium anthraquinone-α-sulfonate
-
Health and Chemical more >
-
Hot Products
- 621-23-8 1,3,5-Trimethoxybenzene
- 636-61-3 D(+)-Malic acid
- 68511-39-7 Poly(oxy-1,2-ethanediyl), .alpha.-sulfo-.omega.-hydroxy-, C12-15-alkyl ethers
- 70415-50-8 6-Chloro-4-hydroxy-2-methyl-2H-thieno[2,3-e]-1,2-thiazine-3-carboxylic acid methyl ester 1,1-dioxide
- 2787-43-1 3-METHYL-1,3-PENTADIENE
- 79982-56-2 osmium bis(2,2'-bipyridine)chloride
- 89059-06-3 1H-PYRROLE-1-PROPANOIC ACID
- 160901-25-7 Sulfonamides,C4-8-alkane, perfluoro, N-ethyl-N-(hydroxyethyl), reaction products with2-ethyl-1-hexanol and polymethylenepolyphenylene isocyanate
- 9003-07-0 1-Propene, homopolymer
- 865-47-4 Potassium tert-butoxide
- 61788-85-0 Ethoxylated hydrogenated castor oil
- 352230-22-9