Measuring binding of protein to gel-bound ligands using magnetic levitation

DOI: 10.1021/ja211788e

Source and publish data:

Journal of the American Chemical Society p. 5637 - 5646 (2012)

Update date:2022-08-02

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Authors:

Shapiro, Nathan D.

Mirica, Katherine A.

Soh, Siowling

Phillips, Scott T.

Taran, Olga

MacE, Charles R.

Shevkoplyas, Sergey S.

Whitesides, George M.

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Article abstract of DOI:10.1021/ja211788e

This paper describes the use of magnetic levitation (MagLev) to measure the association of proteins and ligands. The method starts with diamagnetic gel beads that are functionalized covalently with small molecules (putative ligands). Binding of protein to

Full text of DOI:10.1021/ja211788e

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