Communication
ChemComm
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including an isoform of JNK2 (JNK2a1; 44 kDa), were successfully
labeled using all three probes. JNK1 and JNK3 were not detected
possibly due to their relatively low expression in HeLa cells
(Fig. S8A, ESI†).
Our work herein represents the first reported example in
which live-cell imaging and in situ proteome profiling were
carried out to comprehensively compare covalent and non-
covalent probes sharing related target-binding pharmacophores.
Taking JNK probes as examples, our current view is that properly
designed covalent probes may deliver overwhelmingly better
performance in both profiling and imaging of cellular targets
compared to affinity-based probes. With the successful development
of new JNK probes (JP2-TCO/JP3) and two-photon fluorogenic
reporters (QT1/QT2), they may be used for in situ profiling and
imaging of cellular JNK activities in cells expressing moderate/
high endogenous JNKs.
We thank Dr Hailong Zhang (NUS) for his help with chemical
synthesis. Financial support was provided by the Synthetic
Biology Research & Development Programme (SBP) of National
Research Foundation (NRF), the GSK-EDB Trust Fund (R-143-
000-688-592), MOE-T1 (R-143-000-694-114) of Singapore, the
National Natural Science Foundation of China (21877100,
21708034, 81672508 and 61505076) and the Jiangsu Provincial
Foundation for Distinguished Young Scholars (BK20170041).
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Conflicts of interest
There are no conflicts to declare.
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Chem. Commun.
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